Chemotaxis of cell populations through confined spaces at Single-Cell resolution

Zi Qiu Tong, Eric M. Balzer, Matthew R. Dallas, Wei Chien Hung, Kathleen J. Stebe, Konstantinos Konstantopoulos

Research output: Contribution to journalArticle

Abstract

Cell migration is crucial for both physiological and pathological processes. Current in vitro cell motility assays suffer from various drawbacks, including insufficient temporal and/or optical resolution, or the failure to include a controlled chemotactic stimulus. Here, we address these limitations with a migration chamber that utilizes a self-sustaining chemotactic gradient to induce locomotion through confined environments that emulate physiological settings. Dynamic real-time analysis of both population-scale and single-cell movement are achieved at high resolution. Interior surfaces can be functionalized through adsorption of extracellular matrix components, and pharmacological agents can be administered to cells directly, or indirectly through the chemotactic reservoir. Direct comparison of multiple cell types can be achieved in a single enclosed system to compare inherent migratory potentials. Our novel microfluidic design is therefore a powerful tool for the study of cellular chemotaxis, and is suitable for a wide range of biological and biomedical applications.

Original languageEnglish (US)
Article numbere29211
JournalPloS one
Volume7
Issue number1
DOIs
StatePublished - Jan 18 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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