Abstract
Chemotactic selection is a method by which populations of cells exposed to ligands can be isolated and subsequently cultivated. We used Tetrahymena pyriformis GL cultures selected by chemotactic selection to insulin (10 nM), histamine (0.1 nM) and di-iodotyrosine (T2, 10 nM) to study the phagocytotic capacity under the induction of selector hormones. Our results show a long-lasting link between chemotactically selected cultures and phagocytotic activity. Cells selected to histamine produced the highest phagocytotic activity upon a second exposure to the selector hormone. T2 selection was also strongly effective, however, the phagocytosis stimulation was not specific to the hormone given later. Insulin selected sub-populations had different phagocytotic responses to the control substance itself, whereas histamine selected sub-populations seem to be heterogeneous in the phagocytotic response to histamine. For insulin, the increased endocytotic or metabolic activity was demonstrated by the lack of non-phagocytotic cells. These experiments call attention to the evolutionary role of selection in the later developing receptor-hormone relationship.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 521-530 |
| Number of pages | 10 |
| Journal | Comparative Biochemistry and Physiology - C Toxicology and Pharmacology |
| Volume | 128 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Chemotactic selection
- Histamine
- Insulin
- Phagocytosis
- Tetrahymena
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Health, Toxicology and Mutagenesis
- Pharmacology
Cite this
Chemotactic selection with insulin, di-iodotyrosine and histamine alters the phagocytotic responsiveness of Tetrahymena. / Schiess, Nicoline; Csaba, G.; Kohidai, L.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 128, No. 4, 2001, p. 521-530.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemotactic selection with insulin, di-iodotyrosine and histamine alters the phagocytotic responsiveness of Tetrahymena
AU - Schiess, Nicoline
AU - Csaba, G.
AU - Kohidai, L.
PY - 2001
Y1 - 2001
N2 - Chemotactic selection is a method by which populations of cells exposed to ligands can be isolated and subsequently cultivated. We used Tetrahymena pyriformis GL cultures selected by chemotactic selection to insulin (10 nM), histamine (0.1 nM) and di-iodotyrosine (T2, 10 nM) to study the phagocytotic capacity under the induction of selector hormones. Our results show a long-lasting link between chemotactically selected cultures and phagocytotic activity. Cells selected to histamine produced the highest phagocytotic activity upon a second exposure to the selector hormone. T2 selection was also strongly effective, however, the phagocytosis stimulation was not specific to the hormone given later. Insulin selected sub-populations had different phagocytotic responses to the control substance itself, whereas histamine selected sub-populations seem to be heterogeneous in the phagocytotic response to histamine. For insulin, the increased endocytotic or metabolic activity was demonstrated by the lack of non-phagocytotic cells. These experiments call attention to the evolutionary role of selection in the later developing receptor-hormone relationship.
AB - Chemotactic selection is a method by which populations of cells exposed to ligands can be isolated and subsequently cultivated. We used Tetrahymena pyriformis GL cultures selected by chemotactic selection to insulin (10 nM), histamine (0.1 nM) and di-iodotyrosine (T2, 10 nM) to study the phagocytotic capacity under the induction of selector hormones. Our results show a long-lasting link between chemotactically selected cultures and phagocytotic activity. Cells selected to histamine produced the highest phagocytotic activity upon a second exposure to the selector hormone. T2 selection was also strongly effective, however, the phagocytosis stimulation was not specific to the hormone given later. Insulin selected sub-populations had different phagocytotic responses to the control substance itself, whereas histamine selected sub-populations seem to be heterogeneous in the phagocytotic response to histamine. For insulin, the increased endocytotic or metabolic activity was demonstrated by the lack of non-phagocytotic cells. These experiments call attention to the evolutionary role of selection in the later developing receptor-hormone relationship.
KW - Chemotactic selection
KW - Histamine
KW - Insulin
KW - Phagocytosis
KW - Tetrahymena
UR - http://www.scopus.com/inward/record.url?scp=0035066640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035066640&partnerID=8YFLogxK
U2 - 10.1016/S1532-0456(01)00169-7
DO - 10.1016/S1532-0456(01)00169-7
M3 - Article
VL - 128
SP - 521
EP - 530
JO - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology
JF - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology
SN - 0742-8413
IS - 4
ER -