TY - JOUR
T1 - Chemokine-independent in vitro resistance to human immunodeficiency virus (HIV-1) correlating with low viremia in long-term and recently infected HIV-1-positive persons
AU - Schwartz, David H.
AU - Castillo, Renan C.
AU - Arango-Jaramillo, Silvio
AU - Sharma, Usha K.
AU - Song, Hai Feng
AU - Sridharan, Gopalan
N1 - Funding Information:
Received 2 December 1996; revised 2 June 1997. Presented in part: XIth International Conference on AIDS, Vancouver, Canada, July 1996 (abstract A.1117). Informed consent was obtained from all subjects participating in this study. This study conformed to human experimentation guidelines of the US Department of Health and Human Services and was approved by the Johns Hopkins University Institutional Review Board. Financial support: NIH (AI-45207) and Immuno AG, Vienna. Reprints or correspondence: David H. Schwartz, Dept. of Molecular Microbiology and Immunology, Johns Hopkins School of Hygiene and Public Health, 615 N. Wolfe St., Baltimore, MD 21205. * Christian Medical College, Bangalore, India (G.S.); Medical Microbiology and Immunology, University of Maryland, Baltimore (H.F.S.).
PY - 1997
Y1 - 1997
N2 - Chemokines have been implicated as protective factors against human immunodeficiency virus (HIV) infection, competing for binding to receptors that also function as coreceptors for HIV. In this study of HIV-positive donors, peripheral blood mononuclear cell (PBMC) culture resistance to endogenous and exogenous HIV correlated with low plasma viremia and high in vitro RANTES production. However, resistant cells were not rendered susceptible by neutralization of C-C chemokines, and addition of C-C chemokines did not consistently suppress endogenous virus or exogenous HIV- 1(MN). In contrast, CD8 T cell depletion markedly decreased the frequency of resistant cultures without reducing C-C chemokine production. Among newly infected persons, half exhibited phenotype switching from preinfection susceptibility to postinfection resistance, suggesting that genetically predetermined constitutive cytokine production or allelic receptor expression are not generally responsible for in vitro resistance and nonprogression.
AB - Chemokines have been implicated as protective factors against human immunodeficiency virus (HIV) infection, competing for binding to receptors that also function as coreceptors for HIV. In this study of HIV-positive donors, peripheral blood mononuclear cell (PBMC) culture resistance to endogenous and exogenous HIV correlated with low plasma viremia and high in vitro RANTES production. However, resistant cells were not rendered susceptible by neutralization of C-C chemokines, and addition of C-C chemokines did not consistently suppress endogenous virus or exogenous HIV- 1(MN). In contrast, CD8 T cell depletion markedly decreased the frequency of resistant cultures without reducing C-C chemokine production. Among newly infected persons, half exhibited phenotype switching from preinfection susceptibility to postinfection resistance, suggesting that genetically predetermined constitutive cytokine production or allelic receptor expression are not generally responsible for in vitro resistance and nonprogression.
UR - http://www.scopus.com/inward/record.url?scp=0030773736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030773736&partnerID=8YFLogxK
U2 - 10.1086/514109
DO - 10.1086/514109
M3 - Article
C2 - 9359715
AN - SCOPUS:0030773736
SN - 0022-1899
VL - 176
SP - 1168
EP - 1174
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -