TY - JOUR
T1 - Chemiluminescence from rat alveolar macrophages following induction of phospholipidosis with chlorphentermine
AU - Reasor, Mark J.
AU - Koshut, Roberta A.
AU - McNulty, Michael J.
AU - Trush, Michael A.
N1 - Funding Information:
The authors are grateful to Mr. Robert J. Koshut for conducting the polynomial regression analysis and transforming the temporal chemiluminescence data into the estimated continuous response curves. The assistance of Mrs. Cheryl Zambito and Mrs. Ellen Teicheira during the preparation of this manuscript is appreciated. We wish to thank Warner-Chilcott for the generous gift of chlorphentermine. This research was supported by a Pharmaceutical Manufacturers Association Foundation Research Starter Grant, a West Virginia University Senate Grant, by Biomedical Support Grant SO7 RR0543317, and by the West Virginia University Medical Corporation.
PY - 1980/3/15
Y1 - 1980/3/15
N2 - Rats were treated with chlorphentermine (30 mg/kg) 5 days a week for 4 weeks during which time a phospholipidosis developed in the alveolar macrophages of the lung. Compared to control macrophages, an enhanced chemiluminescence (CL), with altered kinetics, was generated when cells from treated rats were challenged with zymosan. The role of reactive oxygen species, particularly superoxide anion, in the increased CL was studied. Superoxide dismutase inhibited the CL from control cells correspondingly more than with lipidotic cells indicating processes other than the release of superoxide anion from the cells were involved in the enhanced CL response. While total oxygen consumption was similar for both cell populations, lipidotic cells released less superoxide anion during challenge than controls. Concomitantly, lipidotic cells showed an increase in lipid peroxidation. These results are consistent with an explanation that rather than being released by the cell, superoxide anion may become involved in a secondary reaction within the cell such as lipid peroxidation which is then reflected in the altered CL response.
AB - Rats were treated with chlorphentermine (30 mg/kg) 5 days a week for 4 weeks during which time a phospholipidosis developed in the alveolar macrophages of the lung. Compared to control macrophages, an enhanced chemiluminescence (CL), with altered kinetics, was generated when cells from treated rats were challenged with zymosan. The role of reactive oxygen species, particularly superoxide anion, in the increased CL was studied. Superoxide dismutase inhibited the CL from control cells correspondingly more than with lipidotic cells indicating processes other than the release of superoxide anion from the cells were involved in the enhanced CL response. While total oxygen consumption was similar for both cell populations, lipidotic cells released less superoxide anion during challenge than controls. Concomitantly, lipidotic cells showed an increase in lipid peroxidation. These results are consistent with an explanation that rather than being released by the cell, superoxide anion may become involved in a secondary reaction within the cell such as lipid peroxidation which is then reflected in the altered CL response.
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U2 - 10.1016/0041-008X(80)90344-0
DO - 10.1016/0041-008X(80)90344-0
M3 - Article
C2 - 7368220
AN - SCOPUS:0018882146
SN - 0041-008X
VL - 52
SP - 497
EP - 506
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 3
ER -