Chemiluminescence associated with phagocytosis of foreign particles in rabbit alveolar macrophages

P. R. Miles, Ping Lee, Michael A. Trush, Knox Van Dyke

Research output: Contribution to journalArticle

Abstract

Rabbit alveolar macrophages exhibit a chemiluminescent response which is associated with phagocytosis of zymosan and polystyrene-butadiene particles. The chemiluminescence reaches a peak in 15 to 25 minutes and then gradually diminishes over the next 1 to 3 hours. During the time of maximal light emission there appears to be no actual uptake of particles, but the response is dependent upon the particle concentration. The metabolic inhibitor, DNP (2,4-dinitrophenol), causes a rapid inhibition of the chemiluminescent response. The addition of ATP to the medium prior to exposure of the cells to particles causes the chemiluminescent response to be greatly diminished, i.e., 0.3mM ATP virtually abolishes the response. These experiments suggest that some metabolic response of the cell to phagocytosis is responsible for the chemiluminescence.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalLife Sciences
Volume20
Issue number1
DOIs
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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