Chemical Synthesis of Oligodeoxyribonucleotides on the Polystyrene Polymer Support

Srinivasan Chandrasegaran, Lou‐Sing ‐S Kan

Research output: Contribution to journalArticle

Abstract

The synthesis of a decaeoxyribonucleotide, d‐CpCpApApTpTpTpTpGpG, as an example of DNA synthesis on a polystyrene polymer support using the phosphotriesters is described. The procedures for the recovery of the unreacted protected nucleoside phosphotriesters are also included. All operations and reactions were carried out in a 4 ml polypropylene Bio‐Rad Econo column fitted with a 3‐way teflon valve and a rubber septum. The average yield of each monomer addition is 96%. The decamer was obtained in high purity with an isolated yield of 56%. The purified decamer was characterized using a modified Maxam‐Gilbert procedure.

Original languageEnglish (US)
Pages (from-to)359-364
Number of pages6
JournalJournal of the Chinese Chemical Society
Volume32
Issue number3
DOIs
StatePublished - 1985

Keywords

  • decaeoxyribonucleotide
  • Oligodeoxyribonucleotide synthesis
  • solid phase synthesis

ASJC Scopus subject areas

  • Chemistry(all)

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