Abstract
The synthesis of a decaeoxyribonucleotide, d‐CpCpApApTpTpTpTpGpG, as an example of DNA synthesis on a polystyrene polymer support using the phosphotriesters is described. The procedures for the recovery of the unreacted protected nucleoside phosphotriesters are also included. All operations and reactions were carried out in a 4 ml polypropylene Bio‐Rad Econo column fitted with a 3‐way teflon valve and a rubber septum. The average yield of each monomer addition is 96%. The decamer was obtained in high purity with an isolated yield of 56%. The purified decamer was characterized using a modified Maxam‐Gilbert procedure.
Original language | English (US) |
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Pages (from-to) | 359-364 |
Number of pages | 6 |
Journal | Journal of the Chinese Chemical Society |
Volume | 32 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1985 |
Externally published | Yes |
Keywords
- Oligodeoxyribonucleotide synthesis
- decaeoxyribonucleotide
- solid phase synthesis
ASJC Scopus subject areas
- General Chemistry