Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat

Stephen Gulley; Shannon J. Raboin; Sheryce C. Henley; Wai Chu Chan; Sanjay K. Sharma; Leonard H. Billups; Alton R. Esdaile; Cherese N. Sullivan; Timothy H. Moran; Ayman I. Sayegh

doi:10.1016/j.brainres.2005.09.053

Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat

Stephen Gulley, Shannon J. Raboin, Sheryce C. Henley, Wai Chu Chan, Sanjay K. Sharma, Leonard H. Billups, Alton R. Esdaile, Cherese N. Sullivan, Timothy H. Moran, Ayman I. Sayegh

School of Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.

Original language	English (US)
Pages (from-to)	119-125
Number of pages	7
Journal	Brain research
Volume	1064
Issue number	1-2
DOIs	https://doi.org/10.1016/j.brainres.2005.09.053
State	Published - Dec 7 2005

Keywords

Area postrema
C-Fos
Chemical sympathectomy
Cholecystokinin
Dorsal vagal complex
Enteric nervous system
Guanethidine sulfate
Myenteric plexus
NTS
Vagus

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2005.09.053

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Gulley, S., Raboin, S. J., Henley, S. C., Chan, W. C., Sharma, S. K., Billups, L. H., Esdaile, A. R., Sullivan, C. N., Moran, T. H., & Sayegh, A. I. (2005). Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat. Brain research, 1064(1-2), 119-125. https://doi.org/10.1016/j.brainres.2005.09.053

@article{d2a38f5b7b2747609d581b7c5215f638,

title = "Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat",

abstract = "Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.",

keywords = "Area postrema, C-Fos, Chemical sympathectomy, Cholecystokinin, Dorsal vagal complex, Enteric nervous system, Guanethidine sulfate, Myenteric plexus, NTS, Vagus",

author = "Stephen Gulley and Raboin, {Shannon J.} and Henley, {Sheryce C.} and Chan, {Wai Chu} and Sharma, {Sanjay K.} and Billups, {Leonard H.} and Esdaile, {Alton R.} and Sullivan, {Cherese N.} and Moran, {Timothy H.} and Sayegh, {Ayman I.}",

note = "Funding Information: We thank Drs. Abdullah E. Eljack, Department of Biomedical Sciences, Tuskegee University, Eugene Johnson, Washington University School of Medicine, and Michael F. Wiater, Department of VCAPP, Washington State University, for examining the sympathetic ganglia and providing advice on the guanethidine protocol, respectively. Grants NIH S06/GM08091-31, The Birmingham Racing Commission, and Pfizer Student Fund Program supported this work. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2005",

month = dec,

day = "7",

doi = "10.1016/j.brainres.2005.09.053",

language = "English (US)",

volume = "1064",

pages = "119--125",

journal = "Brain research",

issn = "0006-8993",

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T1 - Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat

AU - Gulley, Stephen

AU - Raboin, Shannon J.

AU - Henley, Sheryce C.

AU - Chan, Wai Chu

AU - Sharma, Sanjay K.

AU - Billups, Leonard H.

AU - Esdaile, Alton R.

AU - Sullivan, Cherese N.

AU - Moran, Timothy H.

AU - Sayegh, Ayman I.

N1 - Funding Information: We thank Drs. Abdullah E. Eljack, Department of Biomedical Sciences, Tuskegee University, Eugene Johnson, Washington University School of Medicine, and Michael F. Wiater, Department of VCAPP, Washington State University, for examining the sympathetic ganglia and providing advice on the guanethidine protocol, respectively. Grants NIH S06/GM08091-31, The Birmingham Racing Commission, and Pfizer Student Fund Program supported this work. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/12/7

Y1 - 2005/12/7

N2 - Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.

AB - Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.

KW - Area postrema

KW - C-Fos

KW - Chemical sympathectomy

KW - Cholecystokinin

KW - Dorsal vagal complex

KW - Enteric nervous system

KW - Guanethidine sulfate

KW - Myenteric plexus

KW - NTS

KW - Vagus

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U2 - 10.1016/j.brainres.2005.09.053

DO - 10.1016/j.brainres.2005.09.053

M3 - Article

C2 - 16298351

AN - SCOPUS:28844490500

SN - 0006-8993

VL - 1064

SP - 119

EP - 125

JO - Brain research

JF - Brain research

IS - 1-2

ER -

Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat

Abstract

Keywords

ASJC Scopus subject areas

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