Chemical sympathectomy attenuates myenteric but not dorsal vagal complex Fos-like immunoreactivity induced by cholecystokinin-8 in the rat

Stephen Gulley, Shannon J. Raboin, Sheryce C. Henley, Wai Chu Chan, Sanjay K. Sharma, Leonard H. Billups, Alton R. Esdaile, Cherese N. Sullivan, Timothy H. Moran, Ayman I. Sayegh

Research output: Contribution to journalArticlepeer-review

Abstract

Vagotomy and capsaicin treatment attenuate dorsal vagal complex (DVC) but not myenteric Fos-like immunoreactivity (Fos-LI) induced by cholecystokinin-8 (CCK-8). The goal of this experiment is to test the role of the sympathetic nervous system in the pathway by which CCK-8 increases myenteric Fos-LI. Adult male Sprague-Dawley rats were pretreated with guanethidine sulfate (40 mg/kg daily for 5 weeks) or vehicle intraperitoneally (IP), and injected with CCK-8 (40 μg/kg) or saline IP. Fos-LI was then quantified in the DVC and the myenteric neurons of the duodenum and jejunum using a diaminobenzidine reaction. Guanethidine pretreatment attenuated myenteric but not DVC Fos-LI induced by CCK-8. These findings demonstrate that sympathetic neurons play a role in mediating the myenteric Fos-LI response to CCK. They also suggest differential mediation of myenteric and DVC responses to CCK.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalBrain research
Volume1064
Issue number1-2
DOIs
StatePublished - Dec 7 2005

Keywords

  • Area postrema
  • C-Fos
  • Chemical sympathectomy
  • Cholecystokinin
  • Dorsal vagal complex
  • Enteric nervous system
  • Guanethidine sulfate
  • Myenteric plexus
  • NTS
  • Vagus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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