TY - JOUR
T1 - Chemical probes and tandem mass spectrometry
T2 - A strategy for the quantitative analysis of proteomes and subproteomes
AU - Zhang, Hui
AU - Yan, Wei
AU - Aebersold, Ruedi
N1 - Funding Information:
We thank Jimmy Eng for Table 1 , and Julian Watts, Sharon Chen and Daniel Martin for proof reading. This work was supported in part by grants from the National Cancer Institute (R33, CA93302), by federal funds from the National Heart, Lung, and Blood Institute, National Institutes of Health, Proteomics Initiative (N01-HV-28179), and National Institute for Drug Abuse (1P30DA01562501) of the National Institutes of Health and by a sponsored research agreement from MacroGenics. The ISB is supported by a generous gift from Merck and CO.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2004/2
Y1 - 2004/2
N2 - Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.
AB - Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.
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U2 - 10.1016/j.cbpa.2003.12.001
DO - 10.1016/j.cbpa.2003.12.001
M3 - Review article
C2 - 15036159
AN - SCOPUS:1042275609
VL - 8
SP - 66
EP - 75
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
IS - 1
ER -