Chemical probes and tandem mass spectrometry: A strategy for the quantitative analysis of proteomes and subproteomes

Hui Zhang, Wei Yan, Ruedi Aebersold

Research output: Contribution to journalReview articlepeer-review

Abstract

Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.

Original languageEnglish (US)
Pages (from-to)66-75
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume8
Issue number1
DOIs
StatePublished - Feb 2004
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint

Dive into the research topics of 'Chemical probes and tandem mass spectrometry: A strategy for the quantitative analysis of proteomes and subproteomes'. Together they form a unique fingerprint.

Cite this