Chemical exchange saturation transfer (CEST) MR technique for in-vivo liver imaging at 3.0 tesla

Shu Zhong Chen, Jing Yuan, Min Deng, Juan Wei, Jinyuan Zhou, Yì Xiáng J. Wáng

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To evaluate Chemical Exchange Saturation Transfer (CEST) MRI for liver imaging at 3.0-T. Materials and methods: Images were acquired at offsets (n = 41, increment = 0.25 ppm) from −5 to 5 ppm using a TSE sequence with a continuous rectangular saturation pulse. Amide proton transfer-weighted (APTw) and GlycoCEST signals were quantified as the asymmetric magnetization transfer ratio (MTRasym) at 3.5 ppm and the total MTRasym integrated from 0.5 to 1.5 ppm, respectively, from the corrected Z-spectrum. Reproducibility was assessed for rats and humans. Eight rats were devoid of chow for 24 hours and scanned before and after fasting. Eleven rats were scanned before and after one-time CCl4 intoxication. Results: For reproducibility, rat liver APTw and GlycoCEST measurements had 95 % limits of agreement of −1.49 % to 1.28 % and −0.317 % to 0.345 %. Human liver APTw and GlycoCEST measurements had 95 % limits of agreement of −0.842 % to 0.899 % and −0.344 % to 0.164 %. After 24 hours, fasting rat liver APTw and GlycoCEST signals decreased from 2.38 ± 0.86 % to 0.67 ± 1.12 % and from 0.34 ± 0.26 % to −0.18 ± 0.37 % respectively (p < 0.05). After CCl4 intoxication rat liver APTw and GlycoCEST signals decreased from 2.46 ± 0.48 % to 1.10 ± 0.77 %, and from 0.34 ± 0.23 % to −0.16 ± 0.51 % respectively (p < 0.05). Conclusion: CEST liver imaging at 3.0-T showed high sensitivity for fasting as well as CCl4 intoxication. Key Points: • CEST MRI of in-vivo liver was demonstrated at clinical 3 T field strength. • After 24-hour fasting, rat liver APTw and GlycoCEST signals decreased significantly. • After CCl4 intoxication both rat liver APTw and GlycoCEST signals decreased significantly. • Good scan–rescan reproducibility of liver CEST MRI was shown in healthy volunteers.

Original languageEnglish (US)
Pages (from-to)1792-1800
Number of pages9
JournalEuropean radiology
Volume26
Issue number6
DOIs
StatePublished - Jun 1 2016

Keywords

  • Amide proton transfer (APT)
  • Chemical exchange saturation transfer (CEST)
  • Glycogen
  • Liver
  • Magnetic resonance imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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