Characterizing novel olfactory receptors expressed in the murine renal cortex

Victoria L. Halperin Kuhns, Jason Sanchez, Dylan C. Sarver, Zoya Khalil, Premraj Rajkumar, Kieren Marr, Jennifer Pluznick

Research output: Contribution to journalArticle

Abstract

The kidney uses specialized G protein-coupled receptors, including olfactory receptors (ORs), to act as sensors of molecules and metabolites. In the present study, we cloned and studied seven renal ORs, which we previously found to be expressed in the murine renal cortex. As most ORs are orphan receptors, our goal was to identify ligands for these ORs in the hope that this will guide future research into their functional roles. We identified novel ligands for two ORs: Olfr558 and Olfr90. For Olfr558, we confirmed activation by previously reported ligands and identified 16 additional carboxylic acids that activated this OR. The strongest activation of Olfr558 was produced by butyric, cyclobutanecarboxylic, isovaleric, 2-methylvaleric, 3-methylvaleric, 4-methylvaleric, and valeric acids. The primary in vivo source of both butyric and isovaleric acids is gut microbial metabolism. We also identified 14 novel ligands that activated Olfr90, the strongest of which were 2-methyl-4-propyl-1,3-oxathiane, 1-octen-3-ol, 2-octanol, and 3-octanol. Interestingly, 8 of these 14 ligands are of fungal origin. We also investigated the tissue distribution of these receptors and found that they are each found in a subset of "nonsensory" tissues. Finally, we examined the putative human orthologs of Olfr558 and Olfr90 and found that the human ortholog of Olfr558 (OR51E1) has a similar ligand profile, indicating that the role of this OR is likely evolutionarily conserved. In summary, we examined seven novel renal ORs and identified new ligands for Olfr558 and Olfr90, which imply that both of these receptors serve to detect metabolites produced by microorganisms.

Original languageEnglish (US)
Pages (from-to)F172-F186
JournalAmerican journal of physiology. Renal physiology
Volume317
Issue number1
DOIs
StatePublished - Jul 1 2019

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Odorant Receptors
Kidney
Ligands
Octanols
Butyrates
Tissue Distribution
G-Protein-Coupled Receptors
Carboxylic Acids

Keywords

  • G protein-coupled receptors
  • microbiota
  • Olfr558
  • Olfr90
  • OR51E1

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Characterizing novel olfactory receptors expressed in the murine renal cortex. / Halperin Kuhns, Victoria L.; Sanchez, Jason; Sarver, Dylan C.; Khalil, Zoya; Rajkumar, Premraj; Marr, Kieren; Pluznick, Jennifer.

In: American journal of physiology. Renal physiology, Vol. 317, No. 1, 01.07.2019, p. F172-F186.

Research output: Contribution to journalArticle

Halperin Kuhns, Victoria L. ; Sanchez, Jason ; Sarver, Dylan C. ; Khalil, Zoya ; Rajkumar, Premraj ; Marr, Kieren ; Pluznick, Jennifer. / Characterizing novel olfactory receptors expressed in the murine renal cortex. In: American journal of physiology. Renal physiology. 2019 ; Vol. 317, No. 1. pp. F172-F186.
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