Characterizing microscopic domains of birefringence in thin tissue sections

S. L. Jacques; A. Moody; J. C. Ramella Roman

doi:10.1117/12.434735

Characterizing microscopic domains of birefringence in thin tissue sections

S. L. Jacques, A. Moody, J. C. Ramella Roman

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	D.D. Duncan, S.L. Jacques, P.C. Johnson
Pages	464-468
Number of pages	5
Volume	4257
DOIs	https://doi.org/10.1117/12.434735
State	Published - 2001
Externally published	Yes
Event	Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical - San Jose, CA, United States Duration: Jan 21 2001 → Jan 24 2001

Other

Other	Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical
Country/Territory	United States
City	San Jose, CA
Period	1/21/01 → 1/24/01

Keywords

Fiber orientation
Liver
Muscle
Polarization
Skin

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.434735

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Jacques, SL, Moody, A & Ramella Roman, JC 2001, Characterizing microscopic domains of birefringence in thin tissue sections. in DD Duncan, SL Jacques & PC Johnson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4257, pp. 464-468, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, San Jose, CA, United States, 1/21/01. https://doi.org/10.1117/12.434735

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AB - A tissue engineering problem that we anticipate will become increasingly of interest is how to grow protein layers and filaments in preferred orientations. For example, the polymerization of monomers into an oriented structure which may exert influence on adherent cells. In this paper, we report on an optical solution using polarized light measurements to probe the structure and orientation of fibers. In particular in this initial study, we measure the fast-axis orientation and retardance of micro-domains in thin sections of liver, muscle, and skin tissues using a polarizing microscope. The size of microdomains of iso-retardance is in the range 10-100 μm, which suggests that optical measurements with laser beams that are on the order of 1-mm in diameter or with unaging cameras with pixels sizes on the order of 100 s of μm will average over several microdomains and consequently complicate interpretation of measurements.

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ER -

Characterizing microscopic domains of birefringence in thin tissue sections

Abstract

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Keywords

ASJC Scopus subject areas

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