Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Marian Garcia-Nuñez, Sara Quero, Maria Lluisa Pedro-Botet, Irene Barrabeig, Josep Avarez, Irene Campoy, Maria Rosa Sala, Noemí Parraga-Niño, Sofia Minguell, Joan Caylà, Lourdes Mateu, Miquel Sabria

Research output: Contribution to journalArticle

Abstract

Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

Original languageEnglish (US)
Pages (from-to)865-875
Number of pages11
JournalFuture Microbiology
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

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Legionella pneumophila
Virulence
Epidemiologic Studies
Monoclonal Antibodies

Keywords

  • clinical isolates
  • Legionella
  • monoclonal antibody
  • sequence-based typing

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Garcia-Nuñez, M., Quero, S., Pedro-Botet, M. L., Barrabeig, I., Avarez, J., Campoy, I., ... Sabria, M. (2016). Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. Future Microbiology, 11(7), 865-875. https://doi.org/10.2217/fmb-2016-0013

Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. / Garcia-Nuñez, Marian; Quero, Sara; Pedro-Botet, Maria Lluisa; Barrabeig, Irene; Avarez, Josep; Campoy, Irene; Sala, Maria Rosa; Parraga-Niño, Noemí; Minguell, Sofia; Caylà, Joan; Mateu, Lourdes; Sabria, Miquel.

In: Future Microbiology, Vol. 11, No. 7, 01.07.2016, p. 865-875.

Research output: Contribution to journalArticle

Garcia-Nuñez, M, Quero, S, Pedro-Botet, ML, Barrabeig, I, Avarez, J, Campoy, I, Sala, MR, Parraga-Niño, N, Minguell, S, Caylà, J, Mateu, L & Sabria, M 2016, 'Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing', Future Microbiology, vol. 11, no. 7, pp. 865-875. https://doi.org/10.2217/fmb-2016-0013
Garcia-Nuñez, Marian ; Quero, Sara ; Pedro-Botet, Maria Lluisa ; Barrabeig, Irene ; Avarez, Josep ; Campoy, Irene ; Sala, Maria Rosa ; Parraga-Niño, Noemí ; Minguell, Sofia ; Caylà, Joan ; Mateu, Lourdes ; Sabria, Miquel. / Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. In: Future Microbiology. 2016 ; Vol. 11, No. 7. pp. 865-875.
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