Abstract
Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 865-875 |
| Number of pages | 11 |
| Journal | Future Microbiology |
| Volume | 11 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2016 |
| Externally published | Yes |
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Keywords
- clinical isolates
- Legionella
- monoclonal antibody
- sequence-based typing
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)
Cite this
Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. / Garcia-Nuñez, Marian; Quero, Sara; Pedro-Botet, Maria Lluisa; Barrabeig, Irene; Avarez, Josep; Campoy, Irene; Sala, Maria Rosa; Parraga-Niño, Noemí; Minguell, Sofia; Caylà, Joan; Mateu, Lourdes; Sabria, Miquel.
In: Future Microbiology, Vol. 11, No. 7, 01.07.2016, p. 865-875.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing
AU - Garcia-Nuñez, Marian
AU - Quero, Sara
AU - Pedro-Botet, Maria Lluisa
AU - Barrabeig, Irene
AU - Avarez, Josep
AU - Campoy, Irene
AU - Sala, Maria Rosa
AU - Parraga-Niño, Noemí
AU - Minguell, Sofia
AU - Caylà, Joan
AU - Mateu, Lourdes
AU - Sabria, Miquel
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.
AB - Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.
KW - clinical isolates
KW - Legionella
KW - monoclonal antibody
KW - sequence-based typing
UR - http://www.scopus.com/inward/record.url?scp=84979497599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979497599&partnerID=8YFLogxK
U2 - 10.2217/fmb-2016-0013
DO - 10.2217/fmb-2016-0013
M3 - Article
C2 - 27357520
AN - SCOPUS:84979497599
VL - 11
SP - 865
EP - 875
JO - Future Microbiology
JF - Future Microbiology
SN - 1746-0913
IS - 7
ER -