Characterization of tumor virus proteins. I. Radioimmunoassay of the P 27 protein of avian viruses

T. Higuchi, J. T. August

Research output: Contribution to journalArticlepeer-review

Abstract

The major structural protein of avian oncornaviruses, a core component of about 2700 daltons, has been measured by radioimmunoassay. The purified protein was labelled with 125Iodine by chloramine T method. The immune serum titer was defined as the highest serum dilution able to precipitate 50% of the labelled antigen present in the system. A standard competition curve was constructed in order to determine the equivalents of protein, in a system with limiting antibody concentration.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalBrazilian Journal of Medical and Biological Research
Volume10
Issue number1
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

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