Characterization of the tyrosine phosphorylation and distribution of dystrobrevin isoforms

Sudha Balasubramanian, Eric T. Fung, Richard L. Huganir

Research output: Contribution to journalArticle

Abstract

Dystrobrevin, a member of the dystrophin family of proteins, was initially identified as a major tyrosine phosphorylated synaptic protein in the electric organ of Torpedo californica. In this paper, we show that the major sites of tyrosine phosphorylation of Torpedo dystrobrevin are within its C-terminus, on Tyr-693 and Tyr-710. Cloning of the mammalian homologue of dystrobrevin has recently shown that this phosphotyrosine containing tail, or PYCT, is subject to alternative splicing. To compare the expression and distribution of PYCT- and PYCT+ splice variants, we generated antibodies against different regions of dystrobrevin. Here we show that the PYCT- isoform of 62 kDa is expressed at high levels in all tissues examined. In contrast, PYCT+ isoforms are expressed primarily in brain and muscle, where they are concentrated at synapses. Moreover, PYCT+ isoforms associate more tightly with the membrane and with syntrophin, another synaptically enriched protein. These results suggest that PYCT+ isoforms of dystrobrevin are specialized components of the dystroglycan complex which render the complex sensitive to regulation by tyrosine kinases. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)133-140
Number of pages8
JournalFEBS Letters
Volume432
Issue number3
DOIs
StatePublished - Aug 7 1998

Keywords

  • Dystrobrevin
  • Neuromuscular junction
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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