Characterization of the oligosaccharides from the invariant chain associated with murine Ia antigens

S. J. Swiedler, Gerald Warren Hart, J. H. Freed

Research output: Contribution to journalArticle

Abstract

The oligosaccharide side chains of the murine invariant chain (I(i)) glycoprotein associated with the Ia antigens have been characterized. Affinity chromatography using a monoclonal antibody column was employed to purify the I-A(k) antigen from lysates of the AKTB-1b B cell lymphoma. The invariant chain isolated by this procedure was subsequently digested with proteases, and the resulting glycopeptides were fractionated by reverse-phase high-pressure liquid chromatography (HPLC). The invariant chain appears to contain two glycosylation sites, both of which carry high-mannose oligosaccharides, each with a restricted size distribution and an average composition of Man6GIcNAc2 as judged by gel filtration and α-mannosidase digestion. These data, together with the observation that the invariant chain cannot be labeled metabolically with 3H-glucosamine, 3H-fucose, or 3H-galactose, allow the conclusion that the murine invariant chain does not contain complex oligosaccharides and is not O-glycosylated during its association with the Ia antigens.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalJournal of Immunology
Volume131
Issue number1
StatePublished - 1983

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Histocompatibility Antigens Class II
Oligosaccharides
Mannosidases
Fucose
Glycopeptides
Glucosamine
B-Cell Lymphoma
Reverse-Phase Chromatography
Mannose
Galactose
Glycosylation
Affinity Chromatography
Gel Chromatography
Digestion
Glycoproteins
Peptide Hydrolases
High Pressure Liquid Chromatography
Monoclonal Antibodies
invariant chain

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of the oligosaccharides from the invariant chain associated with murine Ia antigens. / Swiedler, S. J.; Hart, Gerald Warren; Freed, J. H.

In: Journal of Immunology, Vol. 131, No. 1, 1983, p. 352-358.

Research output: Contribution to journalArticle

Swiedler, S. J. ; Hart, Gerald Warren ; Freed, J. H. / Characterization of the oligosaccharides from the invariant chain associated with murine Ia antigens. In: Journal of Immunology. 1983 ; Vol. 131, No. 1. pp. 352-358.
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