Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis

B. Heym, Y. Zhang, S. Poulet, D. Young, S. T. Cole

Research output: Contribution to journalComment/debatepeer-review

Abstract

The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid.

Original languageEnglish (US)
Pages (from-to)4255-4259
Number of pages5
JournalJournal of bacteriology
Volume175
Issue number13
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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