Characterization of the insulin and transferrin receptors of two cancer cell lines

Yue Hua Tang, Xin Zhang, Shu Li Xia, Nai He Jing, You Min Feng

Research output: Contribution to journalArticlepeer-review

Abstract

The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transforrin is 7.5∼8.0. Seatchard plot and Hill plot analyses show respectively the receptor number of 2.97 × 106/cell and 6.21 × 107/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16 × 10-7M and 7.57 × 10-7M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25 × 105/cell and 1.40 × 105/cell, with kD of 1.38 × 10-9M and 1.66 × 10-8M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.

Original languageEnglish (US)
Number of pages1
JournalActa Biochimica et Biophysica Sinica
Volume28
Issue number4
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • Human ovarian teratocarcinoma-derived cell line PA-1
  • Insulin receptor
  • Mouse breast cancer cell line GR2H6
  • Transferrin receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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