Characterization of the ileal microbiota in rejecting and nonrejecting recipients of small bowel transplants

P. L. Oh, I. Martínez, Y. Sun, J. Walter, D. A. Peterson, D. F. Mercer

Research output: Contribution to journalArticle

Abstract

Small bowel transplantation can be a life-preserving procedure for patients with irreversible intestinal failure. Allograft rejection remains a major source of morbidity and mortality and its accurate diagnosis and treatment are critical. In this study, we used pyrosequencing of 16S ribosomal RNA gene tags to compare the composition of the ileal microbiota present during nonrejection, prerejection and active rejection states in small bowel transplant patients. During episodes of rejection, the proportions of phylum Firmicutes (p <0.001) and the order Lactobacillales (p <0.01) were significantly decreased, while those of the phylum Proteobacteria, especially the family Enterobacteriaceae, were significantly increased (p <0.005). Receiver-operating characteristic analysis revealed that relative proportions of several bacterial taxa in ileal effluents and especially Firmicutes, could be used to discriminate between nonrejection and active rejection. In conclusion, the findings obtained during this study suggest that small bowel transplant rejection is associated with changes in the microbial populations in ileal effluents and support microbiota profiling as a potential diagnostic biomarker of rejection. Future studies should investigate if the dysbiosis that we observed is a cause or a consequence of the rejection process. Analysis of the gut microbial community in the ileostomy output of small bowel transplant patients can discriminate between patients that are or are not rejecting their grafted intestines.

Original languageEnglish (US)
Pages (from-to)753-762
Number of pages10
JournalAmerican Journal of Transplantation
Volume12
Issue number3
DOIs
StatePublished - Mar 2012

Fingerprint

Microbiota
Transplants
Lactobacillales
Dysbiosis
16S Ribosomal RNA
Proteobacteria
Ileostomy
Graft Rejection
Enterobacteriaceae
rRNA Genes
ROC Curve
Intestines
Allografts
Transplantation
Biomarkers
Morbidity
Mortality
Population
Firmicutes
Therapeutics

Keywords

  • Allograft
  • intestinal microbiota
  • monitoring

ASJC Scopus subject areas

  • Transplantation
  • Immunology and Allergy
  • Pharmacology (medical)

Cite this

Characterization of the ileal microbiota in rejecting and nonrejecting recipients of small bowel transplants. / Oh, P. L.; Martínez, I.; Sun, Y.; Walter, J.; Peterson, D. A.; Mercer, D. F.

In: American Journal of Transplantation, Vol. 12, No. 3, 03.2012, p. 753-762.

Research output: Contribution to journalArticle

Oh, P. L. ; Martínez, I. ; Sun, Y. ; Walter, J. ; Peterson, D. A. ; Mercer, D. F. / Characterization of the ileal microbiota in rejecting and nonrejecting recipients of small bowel transplants. In: American Journal of Transplantation. 2012 ; Vol. 12, No. 3. pp. 753-762.
@article{7aff1920f7dc4575bf640014616555ef,
title = "Characterization of the ileal microbiota in rejecting and nonrejecting recipients of small bowel transplants",
abstract = "Small bowel transplantation can be a life-preserving procedure for patients with irreversible intestinal failure. Allograft rejection remains a major source of morbidity and mortality and its accurate diagnosis and treatment are critical. In this study, we used pyrosequencing of 16S ribosomal RNA gene tags to compare the composition of the ileal microbiota present during nonrejection, prerejection and active rejection states in small bowel transplant patients. During episodes of rejection, the proportions of phylum Firmicutes (p <0.001) and the order Lactobacillales (p <0.01) were significantly decreased, while those of the phylum Proteobacteria, especially the family Enterobacteriaceae, were significantly increased (p <0.005). Receiver-operating characteristic analysis revealed that relative proportions of several bacterial taxa in ileal effluents and especially Firmicutes, could be used to discriminate between nonrejection and active rejection. In conclusion, the findings obtained during this study suggest that small bowel transplant rejection is associated with changes in the microbial populations in ileal effluents and support microbiota profiling as a potential diagnostic biomarker of rejection. Future studies should investigate if the dysbiosis that we observed is a cause or a consequence of the rejection process. Analysis of the gut microbial community in the ileostomy output of small bowel transplant patients can discriminate between patients that are or are not rejecting their grafted intestines.",
keywords = "Allograft, intestinal microbiota, monitoring",
author = "Oh, {P. L.} and I. Mart{\'i}nez and Y. Sun and J. Walter and Peterson, {D. A.} and Mercer, {D. F.}",
year = "2012",
month = "3",
doi = "10.1111/j.1600-6143.2011.03860.x",
language = "English (US)",
volume = "12",
pages = "753--762",
journal = "American Journal of Transplantation",
issn = "1600-6135",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Characterization of the ileal microbiota in rejecting and nonrejecting recipients of small bowel transplants

AU - Oh, P. L.

AU - Martínez, I.

AU - Sun, Y.

AU - Walter, J.

AU - Peterson, D. A.

AU - Mercer, D. F.

PY - 2012/3

Y1 - 2012/3

N2 - Small bowel transplantation can be a life-preserving procedure for patients with irreversible intestinal failure. Allograft rejection remains a major source of morbidity and mortality and its accurate diagnosis and treatment are critical. In this study, we used pyrosequencing of 16S ribosomal RNA gene tags to compare the composition of the ileal microbiota present during nonrejection, prerejection and active rejection states in small bowel transplant patients. During episodes of rejection, the proportions of phylum Firmicutes (p <0.001) and the order Lactobacillales (p <0.01) were significantly decreased, while those of the phylum Proteobacteria, especially the family Enterobacteriaceae, were significantly increased (p <0.005). Receiver-operating characteristic analysis revealed that relative proportions of several bacterial taxa in ileal effluents and especially Firmicutes, could be used to discriminate between nonrejection and active rejection. In conclusion, the findings obtained during this study suggest that small bowel transplant rejection is associated with changes in the microbial populations in ileal effluents and support microbiota profiling as a potential diagnostic biomarker of rejection. Future studies should investigate if the dysbiosis that we observed is a cause or a consequence of the rejection process. Analysis of the gut microbial community in the ileostomy output of small bowel transplant patients can discriminate between patients that are or are not rejecting their grafted intestines.

AB - Small bowel transplantation can be a life-preserving procedure for patients with irreversible intestinal failure. Allograft rejection remains a major source of morbidity and mortality and its accurate diagnosis and treatment are critical. In this study, we used pyrosequencing of 16S ribosomal RNA gene tags to compare the composition of the ileal microbiota present during nonrejection, prerejection and active rejection states in small bowel transplant patients. During episodes of rejection, the proportions of phylum Firmicutes (p <0.001) and the order Lactobacillales (p <0.01) were significantly decreased, while those of the phylum Proteobacteria, especially the family Enterobacteriaceae, were significantly increased (p <0.005). Receiver-operating characteristic analysis revealed that relative proportions of several bacterial taxa in ileal effluents and especially Firmicutes, could be used to discriminate between nonrejection and active rejection. In conclusion, the findings obtained during this study suggest that small bowel transplant rejection is associated with changes in the microbial populations in ileal effluents and support microbiota profiling as a potential diagnostic biomarker of rejection. Future studies should investigate if the dysbiosis that we observed is a cause or a consequence of the rejection process. Analysis of the gut microbial community in the ileostomy output of small bowel transplant patients can discriminate between patients that are or are not rejecting their grafted intestines.

KW - Allograft

KW - intestinal microbiota

KW - monitoring

UR - http://www.scopus.com/inward/record.url?scp=84857653737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857653737&partnerID=8YFLogxK

U2 - 10.1111/j.1600-6143.2011.03860.x

DO - 10.1111/j.1600-6143.2011.03860.x

M3 - Article

VL - 12

SP - 753

EP - 762

JO - American Journal of Transplantation

JF - American Journal of Transplantation

SN - 1600-6135

IS - 3

ER -