TY - JOUR
T1 - Characterization of the carbohydrate components of Taenia solium oncosphere proteins and their role in the antigenicity
AU - Arana, Yanina
AU - Verastegui, Manuela
AU - Tuero, Iskra
AU - Grandjean, Louis
AU - Garcia, Hector H.
AU - Gilman, Robert H.
N1 - Funding Information:
Acknowledgments Funding for this project came from the Gates project 23981, International Foundation for Science (IFS) grant B/ 4103-1 and the RG-ER anonymous fund for Tropical Research. We thank Dr. Eric Cossio, Dr. Carlos Carmona, Cecilia Casaravilla, and Dr. Holger Mayta and the administrative help of Ms. Marjory Meza and the technical assistance of MSc. Milagros Zavaleta.
PY - 2013/10
Y1 - 2013/10
N2 - This study examines the carbohydrate composition of Taenia solium whole oncosphere antigens (WOAs), in order to improve the understanding of the antigenicity of the T. solium. Better knowledge of oncosphere antigens is crucial to accurately diagnose previous exposure to T. solium eggs and thus predict the development of neurocysticercosis. A set of seven lectins conjugates with wide carbohydrate specificity were used on parasite fixations and somatic extracts. Lectin fluorescence revealed that d-mannose, d-glucose, d-galactose and N-acetyl-d-galactosamine residues were the most abundant constituents of carbohydrate chains on the surface of T. solium oncosphere. Lectin blotting showed that posttranslational modification with N-glycosylation was abundant while little evidence of O-linked carbohydrates was observed. Chemical oxidation and enzymatic deglycosylation in situ were performed to investigate the immunoreactivity of the carbohydrate moieties. Linearizing or removing the carbohydrate moieties from the protein backbones did not diminish the immunoreactivity of these antigens, suggesting that a substantial part of the host immune response against T. solium oncosphere is directed against the peptide epitopes on the parasite antigens. Finally, using carbohydrate probes, we demonstrated for the first time that the presence of several lectins on the surface of the oncosphere was specific to carbohydrates found in intestinal mucus, suggesting a possible role in initial attachment of the parasite to host cells.
AB - This study examines the carbohydrate composition of Taenia solium whole oncosphere antigens (WOAs), in order to improve the understanding of the antigenicity of the T. solium. Better knowledge of oncosphere antigens is crucial to accurately diagnose previous exposure to T. solium eggs and thus predict the development of neurocysticercosis. A set of seven lectins conjugates with wide carbohydrate specificity were used on parasite fixations and somatic extracts. Lectin fluorescence revealed that d-mannose, d-glucose, d-galactose and N-acetyl-d-galactosamine residues were the most abundant constituents of carbohydrate chains on the surface of T. solium oncosphere. Lectin blotting showed that posttranslational modification with N-glycosylation was abundant while little evidence of O-linked carbohydrates was observed. Chemical oxidation and enzymatic deglycosylation in situ were performed to investigate the immunoreactivity of the carbohydrate moieties. Linearizing or removing the carbohydrate moieties from the protein backbones did not diminish the immunoreactivity of these antigens, suggesting that a substantial part of the host immune response against T. solium oncosphere is directed against the peptide epitopes on the parasite antigens. Finally, using carbohydrate probes, we demonstrated for the first time that the presence of several lectins on the surface of the oncosphere was specific to carbohydrates found in intestinal mucus, suggesting a possible role in initial attachment of the parasite to host cells.
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U2 - 10.1007/s00436-013-3542-9
DO - 10.1007/s00436-013-3542-9
M3 - Article
C2 - 23982308
AN - SCOPUS:84884702383
SN - 0932-0113
VL - 112
SP - 3569
EP - 3578
JO - Parasitology Research
JF - Parasitology Research
IS - 10
ER -