TY - JOUR
T1 - Characterization of the binding of recombinant mouse sperm fertilin α subunit to mouse eggs
T2 - Evidence for function as a cell adhesion molecule in sperm-egg binding
AU - Evans, Janice Perry
AU - Schultz, Richard M.
AU - Kopf, Gregory S.
PY - 1997/7/1
Y1 - 1997/7/1
N2 - Fertilin (previously known as PH-30) is a sperm protein that is a candidate molecule for mediating the binding and fusion of the sperm and egg plasma membranes. Fertilin is a heterodimer, with a β subunit that has a region of homology to the disintegrin family of integrin ligands and an α subunit that has a region of homology to viral fusion peptides. it has been hypothesized that fertilin β and α asubunits mediate the interactions between sperm and egg plasma membranes, namely, binding and fusion, respectively. To address this hypothesis and to examine specifically the role of fertilin α in fertilization, we have expressed the predicted extracellular domain of mouse fertilin α as a bacterial fusion protein with maltose-binding protein. This fusion protein (hereafter referred to as recombinant fertilin α-Ec) binds to the microvillar region of zona pellucida (ZP)-free eggs, the region of the membrane to which sperm bind. This binding is reduced in the absence of divalent cations and is supported by Ca2+, Mg2+, or Mn2+. Eggs that have been treated with chymotrypsin bind less recombinant fertilin α-EC than do untreated eggs, suggesting that a chymotrypsin-sensitive binding site for recombinant fertilin α-EC is present on egg surfaces. Binding to eggs is also affected by the method used to remove the ZP. Finally, recombinant fertilin α-EC inhibitors the binding of sperm to eggs during in vitro fertilization of ZP-free eggs. These data are the first evidence to suggest that fertilin α can function as a cell adhesion molecule during fertilization, mediating the binding of sperm and egg plasma membranes.
AB - Fertilin (previously known as PH-30) is a sperm protein that is a candidate molecule for mediating the binding and fusion of the sperm and egg plasma membranes. Fertilin is a heterodimer, with a β subunit that has a region of homology to the disintegrin family of integrin ligands and an α subunit that has a region of homology to viral fusion peptides. it has been hypothesized that fertilin β and α asubunits mediate the interactions between sperm and egg plasma membranes, namely, binding and fusion, respectively. To address this hypothesis and to examine specifically the role of fertilin α in fertilization, we have expressed the predicted extracellular domain of mouse fertilin α as a bacterial fusion protein with maltose-binding protein. This fusion protein (hereafter referred to as recombinant fertilin α-Ec) binds to the microvillar region of zona pellucida (ZP)-free eggs, the region of the membrane to which sperm bind. This binding is reduced in the absence of divalent cations and is supported by Ca2+, Mg2+, or Mn2+. Eggs that have been treated with chymotrypsin bind less recombinant fertilin α-EC than do untreated eggs, suggesting that a chymotrypsin-sensitive binding site for recombinant fertilin α-EC is present on egg surfaces. Binding to eggs is also affected by the method used to remove the ZP. Finally, recombinant fertilin α-EC inhibitors the binding of sperm to eggs during in vitro fertilization of ZP-free eggs. These data are the first evidence to suggest that fertilin α can function as a cell adhesion molecule during fertilization, mediating the binding of sperm and egg plasma membranes.
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U2 - 10.1006/dbio.1997.8612
DO - 10.1006/dbio.1997.8612
M3 - Article
C2 - 9224677
AN - SCOPUS:0031194395
SN - 0012-1606
VL - 187
SP - 94
EP - 106
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -