Characterization of the allergens of the house dust mite Euroglyphus maynei

Marjorie S. Morgan, Larry G. Arlian, Kathleen C. Barnes, Enrique Fernandez-Caldas

Research output: Contribution to journalArticle

Abstract

Background: The house dost mite Euroglyphus maynei inhabits homes in many parts of the world and is the source of many allergens. Objective: The purpose of this study was to biochemically and immunologically characterize the major allergens of E. maynei. Methods: Proteins in an extract of E. maynei were separated into 20 fractions by using preparative isoelectric focusing. Most proteins and allergens were contained in fractions 7 to 17 with pH of 4.8 to 8.0. The fractions were further characterized by nonreducing sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with the serum of 16 individuals sensitive to E. maynei. Results: Molecular weights and isoelectric points were assigned to 47 IgE- binding proteins in an E. maynei extract, and the frequency of IgE binding to each allergen was determined. Twenty-two of the allergens were recognized by more than 50% of the 16 sera, and all but one of the subjects had IgE that bound to more than 10 allergens (range, 0 to 32). One of the proteins was identified as the allergen Eur m 2. Conclusion: E. maynei is the source of at least 47 individual allergens that have been characterized by molecular weight, isoelectric point, and IgE-binding properties.

Original languageEnglish (US)
Pages (from-to)222-228
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume100
Issue number2
DOIs
Publication statusPublished - 1997

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Keywords

  • Allergens
  • Characterization
  • Euroglyphus maynei
  • House dust mite

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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