Abstract
Background: The house dost mite Euroglyphus maynei inhabits homes in many parts of the world and is the source of many allergens. Objective: The purpose of this study was to biochemically and immunologically characterize the major allergens of E. maynei. Methods: Proteins in an extract of E. maynei were separated into 20 fractions by using preparative isoelectric focusing. Most proteins and allergens were contained in fractions 7 to 17 with pH of 4.8 to 8.0. The fractions were further characterized by nonreducing sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with the serum of 16 individuals sensitive to E. maynei. Results: Molecular weights and isoelectric points were assigned to 47 IgE- binding proteins in an E. maynei extract, and the frequency of IgE binding to each allergen was determined. Twenty-two of the allergens were recognized by more than 50% of the 16 sera, and all but one of the subjects had IgE that bound to more than 10 allergens (range, 0 to 32). One of the proteins was identified as the allergen Eur m 2. Conclusion: E. maynei is the source of at least 47 individual allergens that have been characterized by molecular weight, isoelectric point, and IgE-binding properties.
Original language | English (US) |
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Pages (from-to) | 222-228 |
Number of pages | 7 |
Journal | The Journal of Allergy and Clinical Immunology |
Volume | 100 |
Issue number | 2 |
DOIs | |
State | Published - 1997 |
Keywords
- Allergens
- Characterization
- Euroglyphus maynei
- House dust mite
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology