Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model

Jayashree A. Gokhale, Patricia A. Glenton, Saeed R. Khan

Research output: Contribution to journalArticle

Abstract

Purpose: The role of Tamm-Horsfall protein in calcium oxalate stone formation is controversial. It is unclear whether Tamm-Horsfall protein has a role in crystallization. If it does, does it act as an inhibitor or promoter of crystallization? To elucidate the nature of its involvement we characterized Tamm-Horsfall protein in a rat model of calcium oxalate nephrolithiasis by in vivo and in vitro techniques. Materials and Methods: Calcium oxalate nephrolithiasis was induced in male Sprague-Dawley rats. The amino acid and carbohydrate composition of Tamm-Horsfall protein from normal rats and those with nephrolithiasis was determined. The Tamm-Horsfall protein gene and protein expression in the kidneys were examined by in situ hybridization and immunohistochemistry. Furthermore, the interaction of Tamm-Horsfall protein and calcium oxalate crystals was assessed by an in vitro crystal aggregation assay. Results: Tamm-Horsfall protein from rats with nephrolithiasis was biochemically similar to that from normal rats. Although Tamm-Horsfall protein was associated with crystal deposits in the renal papillae of rats with nephrolithiasis, Tamm-Horsfall protein messenger RNA expression in the kidneys remained unchanged. In each group Tamm-Horsfall protein inhibited calcium oxalate crystal aggregation by 47%, indicating no change in functional capabilities. Conclusions: The results of this study indicate that urinary excretion, and the biochemical nature and functional capabilities of Tamm-Horsfall protein remain unchanged during experimental calcium oxalate nephrolithiasis. Although staining for Tamm-Horsfall protein was evident in the papillae of rats with nephrolithiasis, the site of Tamm-Horsfall protein synthesis remained cells of the thick ascending limbs of the loop of Henle.

Original languageEnglish (US)
Pages (from-to)1492-1497
Number of pages6
JournalJournal of Urology
Volume166
Issue number4
StatePublished - 2001
Externally publishedYes

Fingerprint

Uromodulin
Nephrolithiasis
Calcium Oxalate
Crystallization
Kidney
Loop of Henle
In Situ Hybridization
Sprague Dawley Rats

Keywords

  • Calcium oxalate
  • Crystallization
  • Kidney
  • Kidney calculi
  • Rats
  • Sprague-Dawley

ASJC Scopus subject areas

  • Urology

Cite this

Gokhale, J. A., Glenton, P. A., & Khan, S. R. (2001). Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model. Journal of Urology, 166(4), 1492-1497.

Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model. / Gokhale, Jayashree A.; Glenton, Patricia A.; Khan, Saeed R.

In: Journal of Urology, Vol. 166, No. 4, 2001, p. 1492-1497.

Research output: Contribution to journalArticle

Gokhale, JA, Glenton, PA & Khan, SR 2001, 'Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model', Journal of Urology, vol. 166, no. 4, pp. 1492-1497.
Gokhale, Jayashree A. ; Glenton, Patricia A. ; Khan, Saeed R. / Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model. In: Journal of Urology. 2001 ; Vol. 166, No. 4. pp. 1492-1497.
@article{2ccd6a53961f4a09b0badeaefab023c2,
title = "Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model",
abstract = "Purpose: The role of Tamm-Horsfall protein in calcium oxalate stone formation is controversial. It is unclear whether Tamm-Horsfall protein has a role in crystallization. If it does, does it act as an inhibitor or promoter of crystallization? To elucidate the nature of its involvement we characterized Tamm-Horsfall protein in a rat model of calcium oxalate nephrolithiasis by in vivo and in vitro techniques. Materials and Methods: Calcium oxalate nephrolithiasis was induced in male Sprague-Dawley rats. The amino acid and carbohydrate composition of Tamm-Horsfall protein from normal rats and those with nephrolithiasis was determined. The Tamm-Horsfall protein gene and protein expression in the kidneys were examined by in situ hybridization and immunohistochemistry. Furthermore, the interaction of Tamm-Horsfall protein and calcium oxalate crystals was assessed by an in vitro crystal aggregation assay. Results: Tamm-Horsfall protein from rats with nephrolithiasis was biochemically similar to that from normal rats. Although Tamm-Horsfall protein was associated with crystal deposits in the renal papillae of rats with nephrolithiasis, Tamm-Horsfall protein messenger RNA expression in the kidneys remained unchanged. In each group Tamm-Horsfall protein inhibited calcium oxalate crystal aggregation by 47{\%}, indicating no change in functional capabilities. Conclusions: The results of this study indicate that urinary excretion, and the biochemical nature and functional capabilities of Tamm-Horsfall protein remain unchanged during experimental calcium oxalate nephrolithiasis. Although staining for Tamm-Horsfall protein was evident in the papillae of rats with nephrolithiasis, the site of Tamm-Horsfall protein synthesis remained cells of the thick ascending limbs of the loop of Henle.",
keywords = "Calcium oxalate, Crystallization, Kidney, Kidney calculi, Rats, Sprague-Dawley",
author = "Gokhale, {Jayashree A.} and Glenton, {Patricia A.} and Khan, {Saeed R.}",
year = "2001",
language = "English (US)",
volume = "166",
pages = "1492--1497",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Characterization of Tamm-Horsfall protein in a rat nephrolithiasis model

AU - Gokhale, Jayashree A.

AU - Glenton, Patricia A.

AU - Khan, Saeed R.

PY - 2001

Y1 - 2001

N2 - Purpose: The role of Tamm-Horsfall protein in calcium oxalate stone formation is controversial. It is unclear whether Tamm-Horsfall protein has a role in crystallization. If it does, does it act as an inhibitor or promoter of crystallization? To elucidate the nature of its involvement we characterized Tamm-Horsfall protein in a rat model of calcium oxalate nephrolithiasis by in vivo and in vitro techniques. Materials and Methods: Calcium oxalate nephrolithiasis was induced in male Sprague-Dawley rats. The amino acid and carbohydrate composition of Tamm-Horsfall protein from normal rats and those with nephrolithiasis was determined. The Tamm-Horsfall protein gene and protein expression in the kidneys were examined by in situ hybridization and immunohistochemistry. Furthermore, the interaction of Tamm-Horsfall protein and calcium oxalate crystals was assessed by an in vitro crystal aggregation assay. Results: Tamm-Horsfall protein from rats with nephrolithiasis was biochemically similar to that from normal rats. Although Tamm-Horsfall protein was associated with crystal deposits in the renal papillae of rats with nephrolithiasis, Tamm-Horsfall protein messenger RNA expression in the kidneys remained unchanged. In each group Tamm-Horsfall protein inhibited calcium oxalate crystal aggregation by 47%, indicating no change in functional capabilities. Conclusions: The results of this study indicate that urinary excretion, and the biochemical nature and functional capabilities of Tamm-Horsfall protein remain unchanged during experimental calcium oxalate nephrolithiasis. Although staining for Tamm-Horsfall protein was evident in the papillae of rats with nephrolithiasis, the site of Tamm-Horsfall protein synthesis remained cells of the thick ascending limbs of the loop of Henle.

AB - Purpose: The role of Tamm-Horsfall protein in calcium oxalate stone formation is controversial. It is unclear whether Tamm-Horsfall protein has a role in crystallization. If it does, does it act as an inhibitor or promoter of crystallization? To elucidate the nature of its involvement we characterized Tamm-Horsfall protein in a rat model of calcium oxalate nephrolithiasis by in vivo and in vitro techniques. Materials and Methods: Calcium oxalate nephrolithiasis was induced in male Sprague-Dawley rats. The amino acid and carbohydrate composition of Tamm-Horsfall protein from normal rats and those with nephrolithiasis was determined. The Tamm-Horsfall protein gene and protein expression in the kidneys were examined by in situ hybridization and immunohistochemistry. Furthermore, the interaction of Tamm-Horsfall protein and calcium oxalate crystals was assessed by an in vitro crystal aggregation assay. Results: Tamm-Horsfall protein from rats with nephrolithiasis was biochemically similar to that from normal rats. Although Tamm-Horsfall protein was associated with crystal deposits in the renal papillae of rats with nephrolithiasis, Tamm-Horsfall protein messenger RNA expression in the kidneys remained unchanged. In each group Tamm-Horsfall protein inhibited calcium oxalate crystal aggregation by 47%, indicating no change in functional capabilities. Conclusions: The results of this study indicate that urinary excretion, and the biochemical nature and functional capabilities of Tamm-Horsfall protein remain unchanged during experimental calcium oxalate nephrolithiasis. Although staining for Tamm-Horsfall protein was evident in the papillae of rats with nephrolithiasis, the site of Tamm-Horsfall protein synthesis remained cells of the thick ascending limbs of the loop of Henle.

KW - Calcium oxalate

KW - Crystallization

KW - Kidney

KW - Kidney calculi

KW - Rats

KW - Sprague-Dawley

UR - http://www.scopus.com/inward/record.url?scp=0034821415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034821415&partnerID=8YFLogxK

M3 - Article

C2 - 11547119

AN - SCOPUS:0034821415

VL - 166

SP - 1492

EP - 1497

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 4

ER -