Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes

Andrew M. Lew, Andrew Mark Pardoll, W. Lee Maloy, B. J. Fowlkes, Ada Kruisbeek, Shi Fey Cheng, Ronald N. Germain, Jeffrey A. Bluestone, Ronald H. Schwartz, John E. Coligan

Research output: Contribution to journalArticle

Abstract

While much information exists about the structure and function of the clonally distributed T cell receptor (TCR) αβ heterodimer, little is known about the γ protein, the product of a third rearranging TCR gene. An antiserum to a carboxyl-terminal peptide common to several of the murine gamma chain constant regions and a monoclonal antibody to the murine T3 complex were used to identify products of this TCR gene family in a subpopulation of Lyt2-, L3T4- thymocytes. This subpopulation does not express TCRα or full-length TCRβ messenger RNA. The gamma chain is a 35-kilodalton (kD) protein that is disulfide-bonded to a 45-kD partner and is associated with the T3 complex. Analysis of the glycosylation pattern of this thymic gamma chain revealed that the major variable region gamma (Vγ) gene transcribed in activated peripheral T cells is absent from this subpopulation. The cells that bear this second T cell receptor may therefore represent a distinct lineage differentiating within the thymus.

Original languageEnglish (US)
Pages (from-to)1401-1405
Number of pages5
JournalScience
Volume234
Issue number4782
StatePublished - 1986

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Antigen Receptors, T-Cell, gamma-delta
Thymocytes
T-Cell Antigen Receptor
CD3 Antigens
T-Cell Receptor Genes
Glycosylation
Disulfides
Thymus Gland
Immune Sera
Proteins
Monoclonal Antibodies
T-Lymphocytes
Messenger RNA
Peptides
Genes

ASJC Scopus subject areas

  • General

Cite this

Lew, A. M., Pardoll, A. M., Maloy, W. L., Fowlkes, B. J., Kruisbeek, A., Cheng, S. F., ... Coligan, J. E. (1986). Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. Science, 234(4782), 1401-1405.

Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. / Lew, Andrew M.; Pardoll, Andrew Mark; Maloy, W. Lee; Fowlkes, B. J.; Kruisbeek, Ada; Cheng, Shi Fey; Germain, Ronald N.; Bluestone, Jeffrey A.; Schwartz, Ronald H.; Coligan, John E.

In: Science, Vol. 234, No. 4782, 1986, p. 1401-1405.

Research output: Contribution to journalArticle

Lew, AM, Pardoll, AM, Maloy, WL, Fowlkes, BJ, Kruisbeek, A, Cheng, SF, Germain, RN, Bluestone, JA, Schwartz, RH & Coligan, JE 1986, 'Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes', Science, vol. 234, no. 4782, pp. 1401-1405.
Lew AM, Pardoll AM, Maloy WL, Fowlkes BJ, Kruisbeek A, Cheng SF et al. Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. Science. 1986;234(4782):1401-1405.
Lew, Andrew M. ; Pardoll, Andrew Mark ; Maloy, W. Lee ; Fowlkes, B. J. ; Kruisbeek, Ada ; Cheng, Shi Fey ; Germain, Ronald N. ; Bluestone, Jeffrey A. ; Schwartz, Ronald H. ; Coligan, John E. / Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. In: Science. 1986 ; Vol. 234, No. 4782. pp. 1401-1405.
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