Characterization of susceptible chiasma configurations that increase the risk for maternal nondisjunction of chromosome 21

Neil E. Lamb, Eleanor Feingold, Amanda Savage, Dimitrios Avramopoulos, Sallie Freeman, Yuanchao Gu, Anni Hallberg, Jane Hersey, Georgia Karadima, Dorothy Pettay, Denise Saker, Joe Shen, Lisa Taft, Margareta Mikkelsen, Michael B. Petersen, Terry Hassold, Stephanie L. Sherman

Research output: Contribution to journalArticle

Abstract

Recent studies of trisomy 21 have shown that altered levels of recombination are associated with maternal non-disjunction occurring at both meiosis I (MI) and meiosis II (MII). To comprehend better the association of recombination with nondisjunction, an understanding of the pattern of meiotic exchange, i.e. the exchange of genetic material at the four-strand stage during prophase, is required. We examined this underlying exchange pattern to determine if specific meiotic configurations are associated with a higher risk of non-disjunction than others. We examined the crossover frequencies of chromosome 21 for three populations: (i) normal female meiotic events; (ii) meiotic events leading to MI non-disjunction; and (iii) those leading to MII non-disjunction. From these crossover frequencies, we estimated the array of meiotic tetrads that produced the observed crossovers. Using this approach, we found that nearly one-half of MI errors were estimated to be achiasmate. The majority of the remaining MI bivalents had exchanges that clustered at the telomere. In contrast, exchanges occurring among MII cases clustered at the pericentromeric region of the chromosome. Unlike the single exchange distributions, double exchanges from the non-disjoined populations seemed to approximate the distribution in the normal population. These data suggest that the location of certain exchanges makes a tetrad susceptible to non-disjunction. Specifically, this susceptibility is associated with the distance between the centromere and closest exchange. This result challenges the widely held concept that events occurring at MII are largely independent of events occurring at MI, and suggests that all non-disjunction events may be initiated during MI and simply resolved at either of the two meiotic stages.

Original languageEnglish (US)
Pages (from-to)1391-1399
Number of pages9
JournalHuman Molecular Genetics
Volume6
Issue number9
DOIs
StatePublished - Sep 1997
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this

Lamb, N. E., Feingold, E., Savage, A., Avramopoulos, D., Freeman, S., Gu, Y., Hallberg, A., Hersey, J., Karadima, G., Pettay, D., Saker, D., Shen, J., Taft, L., Mikkelsen, M., Petersen, M. B., Hassold, T., & Sherman, S. L. (1997). Characterization of susceptible chiasma configurations that increase the risk for maternal nondisjunction of chromosome 21. Human Molecular Genetics, 6(9), 1391-1399. https://doi.org/10.1093/hmg/6.9.1391