TY - JOUR
T1 - Characterization of supercoiled nucleoprotein complexes released from detergent-treated vaccinia virions
AU - Soloski, M. J.
AU - Holowczak, J. A.
PY - 1981
Y1 - 1981
N2 - Treatment of vaccinia virions with 1% sodium dodecyl sulfate in the absence of reducing agents resulted in the release of subviral particles termed 'subnucleoids', which contained viral DNA in combination with four polypeptides with molecular weights of 90,000, 68,000, 58,000, and 10,000. Biochemical and electron microscopic studies showed that viral DNA in combination with these polypeptides was maintained in a superhelical configuration. When subnucleoids were 'fixed' with glutaraldehyde and formaldehyde and then examined by electron microscopy, spherical particles were observed, in which the supercoiled DNA was folded into globular structures that were 20 to 60 nm in diameter and were interconnected by DNA-protein fibers resembling the nucleosome structures described for eucaryotic chromatin.
AB - Treatment of vaccinia virions with 1% sodium dodecyl sulfate in the absence of reducing agents resulted in the release of subviral particles termed 'subnucleoids', which contained viral DNA in combination with four polypeptides with molecular weights of 90,000, 68,000, 58,000, and 10,000. Biochemical and electron microscopic studies showed that viral DNA in combination with these polypeptides was maintained in a superhelical configuration. When subnucleoids were 'fixed' with glutaraldehyde and formaldehyde and then examined by electron microscopy, spherical particles were observed, in which the supercoiled DNA was folded into globular structures that were 20 to 60 nm in diameter and were interconnected by DNA-protein fibers resembling the nucleosome structures described for eucaryotic chromatin.
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U2 - 10.1128/jvi.37.2.770-783.1981
DO - 10.1128/jvi.37.2.770-783.1981
M3 - Article
C2 - 7218437
AN - SCOPUS:0019350309
SN - 0022-538X
VL - 37
SP - 770
EP - 783
JO - Journal of virology
JF - Journal of virology
IS - 2
ER -