Characterization of slow conformational dynamics in solids: Dipolar CODEX

Wenbo Li, Ann E. McDermott

Research output: Contribution to journalArticlepeer-review

Abstract

A solid state NMR experiment is introduced for probing relatively slow conformational exchange, based on dephasing and refocusing dipolar couplings. The method is closely related to the previously described Centerband-Only Detection of Exchange or CODEX experiment. The use of dipolar couplings for this application is advantageous because their values are known a priori from molecular structures, and their orientations and reorientations relate in a simple way to molecular geometry and motion. Furthermore the use of dipolar couplings in conjunction with selective isotopic enrichment schemes is consistent with selection for unique sites in complex biopolymers. We used this experiment to probe the correlation time for the motion of 13C, 15N enriched urea molecules within their crystalline lattice.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalJournal of Biomolecular NMR
Volume45
Issue number1-2
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • CODEX
  • Chemical exchange
  • Dynamics
  • Magic angle spinning
  • Solid-state NMR
  • Urea

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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