Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever

K. W. Lam, D. S. Burke, M. Siemens, V. Cipperly, C. Y. Li, L. T. Yam

Research output: Contribution to journalArticle

Abstract

Increases of tartrate-resistant acid phosphatase (EC 3.1.3.2) in the serum of children affected by Dengue hemorrhagic fever were detected by a colorimetric method. Immunochemical and electrophoretic methods confirmed that this increased enzyme activity is identical to that of isoenzyme 5b of osteoclasts. Ion-exchange column chromatography separated the serum acid phosphatase into two components with identical antigenicity, substrate specificity, and electrophoretic mobility. They differ in molecular size.

Original languageEnglish (US)
Pages (from-to)2296-2299
Number of pages4
JournalClinical Chemistry
Volume28
Issue number11
StatePublished - 1982
Externally publishedYes

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Severe Dengue
Acid Phosphatase
Electrophoretic mobility
Column chromatography
Ion Exchange Chromatography
Enzyme activity
Osteoclasts
Substrate Specificity
Serum
Isoenzymes
Ion exchange
Substrates
Enzymes
Tartrate-Resistant Acid Phosphatase
tartaric acid

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Lam, K. W., Burke, D. S., Siemens, M., Cipperly, V., Li, C. Y., & Yam, L. T. (1982). Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever. Clinical Chemistry, 28(11), 2296-2299.

Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever. / Lam, K. W.; Burke, D. S.; Siemens, M.; Cipperly, V.; Li, C. Y.; Yam, L. T.

In: Clinical Chemistry, Vol. 28, No. 11, 1982, p. 2296-2299.

Research output: Contribution to journalArticle

Lam, KW, Burke, DS, Siemens, M, Cipperly, V, Li, CY & Yam, LT 1982, 'Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever', Clinical Chemistry, vol. 28, no. 11, pp. 2296-2299.
Lam KW, Burke DS, Siemens M, Cipperly V, Li CY, Yam LT. Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever. Clinical Chemistry. 1982;28(11):2296-2299.
Lam, K. W. ; Burke, D. S. ; Siemens, M. ; Cipperly, V. ; Li, C. Y. ; Yam, L. T. / Characterization of serum acid phosphatase associated with Dengue hemorrhagic fever. In: Clinical Chemistry. 1982 ; Vol. 28, No. 11. pp. 2296-2299.
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