Characterization of RNA-Binding proteins using protein microarrays

Guang Song, Johnathan Neiswinger, Heng Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

RNA-binding proteins (RBPs), along with target RNA, play a vital role in the regulation of cellular processing and are especially important in gene transcription and posttranscriptional regulation. Here, we present a high-throughput method for rapid identification of RBPs for a given RNA using protein microarray technology. This protocol includes preparation of Cy5-labeled RNA probes, probe denaturing and refolding, and an RNA-binding assay as performed on a yeast protein microarray.

Original languageEnglish (US)
Pages (from-to)881-886
Number of pages6
JournalCold Spring Harbor Protocols
Volume2016
Issue number10
DOIs
StatePublished - Oct 1 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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