Characterization of reiterated human DNA with respect to mammalian X chromosome homology

Ethylin Wang Jabs, Stanley F. Wolf, Barbara R Migeon

Research output: Contribution to journalArticle

Abstract

Recombinants containing human repetitive DNA sequences were identified by dot hybridization and classified with respect to presence on the X chromosome and homology to mouse DNA. Using genomic probes that differ in number of X chromosomes, we observed extensive homology between human autosomal and X sequences. Hybridization to genomic probes that differ in species of origin indicate that these reiterated sequences have diverged between mouse and man. Eleven recombinants, each containing a different reiterated sequence(s), were hybridized in situ to metaphase chromosomes of mouse and man. These studies indicate that reiterated DNA which is homologous to the human X chromosome is more similar to DNA of human autosomes than to any murine chromosome. Therefore, it seems that reiterated DNA sequences on the human X chromosome have diverged as much during mammalian evolution as sequences on human autosomes. Moreover, the extensive modification of the original mammalian X has not interferred with the X inactivation process.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalSomatic Cell and Molecular Genetics
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1984

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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