Characterization of recognition sites on bacteriophage HP1c1 DNA which interact with the DNA uptake system of Haemophilus influenzae Rd

Wayne P. Fitzmaurice, Robert C. Benjamin, P. C. Huang, John J. Scocca

Research output: Contribution to journalArticlepeer-review

Abstract

The 32.4-kb genome of the Haemophilus influenzae bacteriophage HP1c1 contains at least twelve sites, each conferring high affinity for the DNA uptake system of transformable H. influenzae Rd. Five of these high-affinity sites have been located and their nucleotide sequences determined. Three sites contained a contiguous 9-bp sequence identical to the first nine residues of the 11-bp site previously identified as conferring high affinity for the H. influenzae transformation receptor to DNA fragments. The remaining two sites contained complete 11-bp sequences. In contrast, an HP1c1 restriction fragment containing a sequence identical to the final nine residues of the 11-bp uptake site exhibits only a low affinity for the DNA uptake system. An 8-bp sequence consisting of the first eight residues of the 11-bp site was 1% as active as the longer, high-affinity sites. Thus the first 9-bp of the 11-bp site are sufficient to direct high-affinity uptake, while the first 8-bp or the distal 9-bp are not. These results provide an initial assessment of the relative contributions of the individual residues constituting the 11-bp site to the apparent affinity of DNA fragments for the receptor of Haemophilus transformation.

Original languageEnglish (US)
Pages (from-to)187-196
Number of pages10
JournalGene
Volume31
Issue number1-3
DOIs
StatePublished - Nov 1984

Keywords

  • Recombinant DNA
  • plasmids
  • specific DNA receptors
  • transformation efficiency

ASJC Scopus subject areas

  • Genetics

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