TY - JOUR
T1 - Characterization of protein-protein interactions using protein microarrays
AU - Paul, Corry
AU - Rho, Heesool
AU - Neiswinger, Johnathan
AU - Zhu, Heng
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Functional protein microarrays allow fast, straightforward, and efficient high-throughput screening of protein-protein interactions. The microarray approach has outpaced other interaction methods, such as yeast two-hybrid screens, in part because of the vast amounts of information that can be obtained during a single assay. This protocol describes how to perform a binding assay for a protein of interest using a proteome microarray composed of thousands of functional, recombinant proteins adhered to a microchip.
AB - Functional protein microarrays allow fast, straightforward, and efficient high-throughput screening of protein-protein interactions. The microarray approach has outpaced other interaction methods, such as yeast two-hybrid screens, in part because of the vast amounts of information that can be obtained during a single assay. This protocol describes how to perform a binding assay for a protein of interest using a proteome microarray composed of thousands of functional, recombinant proteins adhered to a microchip.
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U2 - 10.1101/pdb.prot087965
DO - 10.1101/pdb.prot087965
M3 - Article
C2 - 27698235
AN - SCOPUS:84990053197
SN - 1940-3402
VL - 2016
SP - 877
EP - 880
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -