Characterization of protein-protein interactions by isothermal titration calorimetry

Adrian Velazquez-Campoy, Stephanie A. Leavitt, Ernesto Freire

Research output: Contribution to journalArticle

Abstract

The analysis of protein-protein interactions has attracted the attention of many researchers from both a fundamental point of view and a practical point of view. From a fundamental point of view, the development of an understanding of the signaling events triggered by the interaction of two or more proteins provides key information to elucidate the functioning of many cell processes. From a practical point of view, understanding protein-protein interactions at a quantitative level provides the foundation for the development of antagonists or agonists of those interactions. Isothermal Titration Calorimetry (ITC) is the only technique with the capability of measuring not only binding affinity but the enthalpic and entropic components that define affinity. Over the years, isothermal titration calorimeters have evolved in sensitivity and accuracy. Today, TA Instruments and MicroCal market instruments with the performance required to evaluate protein-protein interactions. In this methods paper, we describe general procedures to analyze heterodimeric (porcine pancreatic trypsin binding to soybean trypsin inhibitor) and homodimeric (bovine pancreaticα-chymotrypsin) protein associations by ITC.

Original languageEnglish (US)
Pages (from-to)183-204
Number of pages22
JournalMethods in molecular biology (Clifton, N.J.)
Volume1278
DOIs
StatePublished - 2015

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Calorimetry
Proteins
Aprotinin
Chymotrypsin
Soybeans
Trypsin
Swine
Research Personnel

Keywords

  • Binding
  • Calorimetry
  • Dimerization
  • Dissociation
  • Protein-protein interaction
  • Thermodynamics
  • Titration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Characterization of protein-protein interactions by isothermal titration calorimetry. / Velazquez-Campoy, Adrian; Leavitt, Stephanie A.; Freire, Ernesto.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1278, 2015, p. 183-204.

Research output: Contribution to journalArticle

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