Characterization of plasmodium lactate dehydrogenase and histidine-rich protein 2 clearance patterns via rapid on-bead detection from a single dried blood spot

Christine F. Markwalter, Lauren E. Gibson, Lwiindi Mudenda, Danielle W. Kimmel, Saidon Mbambara, Philip E Thuma, David W. Wright

Research output: Contribution to journalArticle

Abstract

A rapid, on-bead enzyme-linked immunosorbent assay for Plasmodium lactate dehydrogenase (pLDH) and Plasmodium falciparum histidine-rich protein 2 (HRP2) was adapted for use with dried blood spot (DBS) samples. This assay detected both biomarkers from a single DBS sample with only 45 minutes of total incubation time and detection limits of 600 ± 500 pM (pLDH) and 69 ± 30 pM (HRP2), corresponding to 150 and 24 parasites/µL, respectively. This sensitive and reproducible on-bead detection method was used to quantify pLDH and HRP2 in patient DBS samples from rural Zambia collected at multiple time points after treatment. Biomarker clearance patterns relative to parasite clearance were determined; pLDH clearance followed closely with parasite clearance, whereas most patients maintained detectable levels of HRP2 for 35–52 days after treatment. Furthermore, weak-to-moderate correlations between biomarker concentration and parasite densities were found for both biomarkers. This work demonstrates the utility of the developed assay for epidemiological study and surveillance of malaria.

Original languageEnglish (US)
Pages (from-to)1389-1396
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume98
Issue number5
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

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Plasmodium
L-Lactate Dehydrogenase
Parasites
Biomarkers
Zambia
Plasmodium falciparum
Malaria
Limit of Detection
Epidemiologic Studies
Enzyme-Linked Immunosorbent Assay
histidine-rich proteins
Therapeutics

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Characterization of plasmodium lactate dehydrogenase and histidine-rich protein 2 clearance patterns via rapid on-bead detection from a single dried blood spot. / Markwalter, Christine F.; Gibson, Lauren E.; Mudenda, Lwiindi; Kimmel, Danielle W.; Mbambara, Saidon; Thuma, Philip E; Wright, David W.

In: American Journal of Tropical Medicine and Hygiene, Vol. 98, No. 5, 01.01.2018, p. 1389-1396.

Research output: Contribution to journalArticle

Markwalter, Christine F. ; Gibson, Lauren E. ; Mudenda, Lwiindi ; Kimmel, Danielle W. ; Mbambara, Saidon ; Thuma, Philip E ; Wright, David W. / Characterization of plasmodium lactate dehydrogenase and histidine-rich protein 2 clearance patterns via rapid on-bead detection from a single dried blood spot. In: American Journal of Tropical Medicine and Hygiene. 2018 ; Vol. 98, No. 5. pp. 1389-1396.
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