Characterization of pig vasa homolog gene and specific expression in germ cell lineage

Gab Sang Lee, Hye Soo Kim, So Hyun Lee, Min Soo Kang, Dae Yong Kim, Chang Kyu Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

The vasa gene is known to be an important factor for germ cell development in both invertebrates and vertebrates. In the present study, we cloned the porcine vasa homolog (Pvh, 2,172 bps) and investigated its expression at mRNA and protein levels. The isolated cDNA had deduced 724 amino acid residues with significant homology to mouse (85%) and human (91%)i/asa. In adult tissues, Pvh transcript was restricted to the ovary and testis, and was undetectable in somatic tissues. During preimplantation embryo development, Pvh was transcribed in oocytes and fertilized 2-cell embryos, but not in other preimplantation embryos. In fetal stage, the transcript of Pvh gene was expressed in all fetal stage, except in day 17-18. Immunohistochemical analysis of fetal and adult gonad revealed that the Pvh protein was localized in oocytes and spermatocytes, consistent with mRNA expression. Interestingly, Pvh protein was also observed in proliferating primordial germ cells (PGCs) and freshly isolated PGCs, but not in embryonic germ cells. Our results suggest that Pvh gene can be a useful marker for germ cell development in pigs.

Original languageEnglish (US)
Pages (from-to)320-328
Number of pages9
JournalMolecular reproduction and development
Volume72
Issue number3
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Keywords

  • Embryo
  • Germ cell
  • Pig
  • Vasa

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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