Characterization of P5, a novel NFAT/AP-1 site in the human IL-4 promoter

Thomas F. Burke, Vincenzo Casolaro, Steve N. Georas

Research output: Contribution to journalArticle

Abstract

Interleukin 4 (IL-4) gene expression is controlled at the level of transcription by the complex interactions of multiple factors that bind to a proximal promoter region. Nuclear factor of activated T cells (NFAT) can bind up to five purine-rich sequences in the IL-4 promoter termed the P elements (P0-P4). In this paper, we characterize a novel P element in the upstream region of the human IL-4 promoter that we term P5. P5 shares a core NFAT motif (-353GGAAA-357) and additional sequence similarity with the other P elements and supported strong interactions between the NFATp DNA-binding domain (DBD) and the AP-1 proteins cFos and cJun in DNA-binding assays. Inducibility of the IL-4 promoter was significantly impaired in a reporter construct in which the P5 element was mutated in the context of the full-length promoter. We conclude that P5 represents a novel IL-4 promoter P element that contributes to IL-4 promoter inducibility. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1016-1023
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number3
DOIs
StatePublished - Apr 21 2000

Keywords

  • Cytokines
  • Interleukin 4
  • NFAT
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Characterization of P5, a novel NFAT/AP-1 site in the human IL-4 promoter'. Together they form a unique fingerprint.

  • Cite this