TY - JOUR
T1 - Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation
AU - Zeller, Karen I.
AU - Haggerty, Timothy J.
AU - Barrett, John F.
AU - Guo, Qingbin
AU - Wonsey, Diane R.
AU - Dang, Chi V.
PY - 2001/12/21
Y1 - 2001/12/21
N2 - The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.
AB - The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.
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U2 - 10.1074/jbc.M108506200
DO - 10.1074/jbc.M108506200
M3 - Article
C2 - 11604407
AN - SCOPUS:0035930544
SN - 0021-9258
VL - 276
SP - 48285
EP - 48291
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 51
ER -