Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation

Karen I. Zeller, Timothy J. Haggerty, John F. Barrett, Qingbin Guo, Diane R. Wonsey, Chi V. Dang

Research output: Contribution to journalArticle

Abstract

The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.

Original languageEnglish (US)
Pages (from-to)48285-48291
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number51
DOIs
StatePublished - Dec 21 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Zeller, K. I., Haggerty, T. J., Barrett, J. F., Guo, Q., Wonsey, D. R., & Dang, C. V. (2001). Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation. Journal of Biological Chemistry, 276(51), 48285-48291. https://doi.org/10.1074/jbc.M108506200