Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation

Karen I. Zeller, Timothy J. Haggerty, John F. Barrett, Qingbin Guo, Diane R. Wonsey, Chi V. Dang

Research output: Contribution to journalArticle

Abstract

The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.

Original languageEnglish (US)
Pages (from-to)48285-48291
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number51
StatePublished - Dec 21 2001

Fingerprint

Chromatin Immunoprecipitation
Chromatin
Transcription Factors
Genes
Scanning
myc Genes
Human Genome
Microarrays
Oligonucleotide Array Sequence Analysis
Gene expression
Introns
Sequence Analysis
Binding Sites
nucleophosmin
Genome
Technology
Gene Expression
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zeller, K. I., Haggerty, T. J., Barrett, J. F., Guo, Q., Wonsey, D. R., & Dang, C. V. (2001). Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation. Journal of Biological Chemistry, 276(51), 48285-48291.

Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation. / Zeller, Karen I.; Haggerty, Timothy J.; Barrett, John F.; Guo, Qingbin; Wonsey, Diane R.; Dang, Chi V.

In: Journal of Biological Chemistry, Vol. 276, No. 51, 21.12.2001, p. 48285-48291.

Research output: Contribution to journalArticle

Zeller, KI, Haggerty, TJ, Barrett, JF, Guo, Q, Wonsey, DR & Dang, CV 2001, 'Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation', Journal of Biological Chemistry, vol. 276, no. 51, pp. 48285-48291.
Zeller KI, Haggerty TJ, Barrett JF, Guo Q, Wonsey DR, Dang CV. Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation. Journal of Biological Chemistry. 2001 Dec 21;276(51):48285-48291.
Zeller, Karen I. ; Haggerty, Timothy J. ; Barrett, John F. ; Guo, Qingbin ; Wonsey, Diane R. ; Dang, Chi V. / Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 51. pp. 48285-48291.
@article{d6cfd5fdbc494ccca8806bbec8b983eb,
title = "Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation",
abstract = "The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.",
author = "Zeller, {Karen I.} and Haggerty, {Timothy J.} and Barrett, {John F.} and Qingbin Guo and Wonsey, {Diane R.} and Dang, {Chi V.}",
year = "2001",
month = "12",
day = "21",
language = "English (US)",
volume = "276",
pages = "48285--48291",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "51",

}

TY - JOUR

T1 - Characterization of Nucleophosmin (B23) as a Myc Target by Scanning Chromatin Immunoprecipitation

AU - Zeller, Karen I.

AU - Haggerty, Timothy J.

AU - Barrett, John F.

AU - Guo, Qingbin

AU - Wonsey, Diane R.

AU - Dang, Chi V.

PY - 2001/12/21

Y1 - 2001/12/21

N2 - The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.

AB - The genetic program through which a specific transcription factor regulates a biological response is fundamental to our understanding how instructions in the genome are implemented. The emergence of DNA microarray technology for gene expression analysis has generated vast numbers of target genes resulting from specific transcription factor activity. We use the oncogenic transcription factor c-Myc as proof-of-principle that human genome sequence analysis and scanning of a specific gene by chromatin immunoprecipitation can be coupled to identify target transcription factor binding sequences. We focused on nucleophosmin, also known as B23, which was identified as a candidate Myc-responsive gene from a subtractive hybridization screen, and we found that sequences in intron 1, and not 5′ sequences in the proximal promoter, are bound by c-Myc in vivo. Hence, a scanning chromatin immunoprecipitation (SChIP) strategy is useful in analyzing functional transcription factor-binding sites.

UR - http://www.scopus.com/inward/record.url?scp=0035930544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035930544&partnerID=8YFLogxK

M3 - Article

C2 - 11604407

AN - SCOPUS:0035930544

VL - 276

SP - 48285

EP - 48291

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 51

ER -