Characterization of nuclear morphology and nuclear matrices in ageing human fibroblasts

Kenneth J. Pienta, Robert H. Getzenberg, Donald S. Coffey

Research output: Contribution to journalArticlepeer-review

Abstract

It is believed that the mechanisms for cellular senescence may reside within the genome, however, the changes which occur in the DNA and the surrounding nuclear environment have not been well documented. As the dynamic skeletal framework of the nucleus, the nuclear matrix is poised to play a critical role in the ageing process. The nuclear matrix plays a central role in DNA organization and nuclear structural morphology. The important roles of the nuclear matrix in cell structure and function are demonstrated by its properties of tissue specificity and that it is altered by viral infection, differentiation and carcinogenesis. We therefore undertook a study to investigate the morphologic alterations which occur in ageing nuclei and to determine whether compositional changes in the nuclear matrix occur with age in human skin fibroblasts. We found that as the nucleus increases in size and becomes more round with age, the qualitative pattern of the prominent nuclear matrix proteins does not appear to undergo major changes with age. There do, however, appear to be quantitative alterations in these proteins.

Original languageEnglish (US)
Pages (from-to)13-24
Number of pages12
JournalMechanisms of Ageing and Development
Volume62
Issue number1
DOIs
StatePublished - Jan 1992

Keywords

  • Ageing
  • Nuclear matrix
  • Nuclear morphology

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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