Characterization of NovP and NovN: Completion of Novobiocin Biosynthesis by Sequential Tailoring of the Noviosyl Ring

Caren L.Freel Meyers, Markus Oberthür, Hui Xu, Lutz Heide, Daniel Kahne, Christopher T. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

Two steps forward: The glycosylated aminocoumarin antibiotic novobiocin (1) is produced by. Streptomyces spheroides and inhibits the bacterial type II topoisomerase DNA gyrase. The sugar-tailoring enzymes NovP and NovN that catalyze the final two steps in novobiocin biosynthesis were overproduced in Escherichia coli and characterized as a methyltransferase and carbamoyltransferase, respectively.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalAngewandte Chemie - International Edition
Volume43
Issue number1
DOIs
StatePublished - Dec 22 2004
Externally publishedYes

Keywords

  • Antibiotics
  • Biosynthesis
  • Enzymes
  • Novobiocin
  • Transferases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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