Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI

Takahiro Higuchi, Stephan G. Nekolla, Antanas Jankaukas, Axel W. Weber, Marc C. Huisman, Sybille Reder, Sibylle I. Ziegler, Markus Schwaiger, Frank M. Bengel

Research output: Contribution to journalArticle

Abstract

The combination of small-animal PET and MRI data provides quantitative in vivo insights into cardiac pathophysiology, integrating information on biology and morphology. We sought to determine the feasibility of PET and MRI for the quantification of ischemic injury in the rat model. Methods: Fourteen healthy male Wistar rats were studied with 18F-FDG PET and cine MRI. Myocardial viability was determined in a transmural myocardial infarction model in 12 additional rats, using 18F-FDG PET and delayed-enhancement MRI with gadolinium-diethylenetriaminepentaacetic acid. All PET was acquired with a dedicated small-animal PET system. MRI was performed on a 1.5-T clinical tomograph with a dedicated small-animal electrocardiographic triggering device and a small surface coil. Results: In normal rats, 18F-FDG uptake was homogeneous throughout the left ventricle. The lowest mean uptake of the 18F-FDG was found in the apical regions (79% ± 6.0% of maximum) and the highest uptake was in the anterior wall (93% ± 4.3 % of maximum). Myocardial infarct size as determined by histology correlated well with defects of glucose metabolism obtained with 18F-FDG PET (r = 0.89) and also with delayed-enhancement MRI (r = 0.91). Left ventricular ejection fraction in normal rats measured by cine MRI was 57% ± 5.4% and decreased to 38% ± 12.9% (P <0.001) in the myocardial infarction model. Conclusion: Integrating information from small-animal PET and clinical MRI instrumentation allows for the quantitative assessment of cardiac function and infarct size in the rat model. The MRI measurements of scar can be complemented by metabolic imaging, addressing the extent and severity of ischemic injury and providing endpoints for therapeutic interventions.

Original languageEnglish (US)
Pages (from-to)288-294
Number of pages7
JournalJournal of Nuclear Medicine
Volume48
Issue number2
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Fluorodeoxyglucose F18
Myocardium
Cine Magnetic Resonance Imaging
Myocardial Infarction
Wounds and Injuries
Gadolinium
Stroke Volume
Heart Ventricles
Cicatrix
Wistar Rats
Histology
Glucose
Equipment and Supplies
Acids

Keywords

  • MRI
  • Myocardial infarction
  • Rat
  • Small-animal PET

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Higuchi, T., Nekolla, S. G., Jankaukas, A., Weber, A. W., Huisman, M. C., Reder, S., ... Bengel, F. M. (2007). Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI. Journal of Nuclear Medicine, 48(2), 288-294.

Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI. / Higuchi, Takahiro; Nekolla, Stephan G.; Jankaukas, Antanas; Weber, Axel W.; Huisman, Marc C.; Reder, Sybille; Ziegler, Sibylle I.; Schwaiger, Markus; Bengel, Frank M.

In: Journal of Nuclear Medicine, Vol. 48, No. 2, 01.02.2007, p. 288-294.

Research output: Contribution to journalArticle

Higuchi, T, Nekolla, SG, Jankaukas, A, Weber, AW, Huisman, MC, Reder, S, Ziegler, SI, Schwaiger, M & Bengel, FM 2007, 'Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI', Journal of Nuclear Medicine, vol. 48, no. 2, pp. 288-294.
Higuchi T, Nekolla SG, Jankaukas A, Weber AW, Huisman MC, Reder S et al. Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI. Journal of Nuclear Medicine. 2007 Feb 1;48(2):288-294.
Higuchi, Takahiro ; Nekolla, Stephan G. ; Jankaukas, Antanas ; Weber, Axel W. ; Huisman, Marc C. ; Reder, Sybille ; Ziegler, Sibylle I. ; Schwaiger, Markus ; Bengel, Frank M. / Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI. In: Journal of Nuclear Medicine. 2007 ; Vol. 48, No. 2. pp. 288-294.
@article{079c3b010b1242038344075471fbbfdb,
title = "Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI",
abstract = "The combination of small-animal PET and MRI data provides quantitative in vivo insights into cardiac pathophysiology, integrating information on biology and morphology. We sought to determine the feasibility of PET and MRI for the quantification of ischemic injury in the rat model. Methods: Fourteen healthy male Wistar rats were studied with 18F-FDG PET and cine MRI. Myocardial viability was determined in a transmural myocardial infarction model in 12 additional rats, using 18F-FDG PET and delayed-enhancement MRI with gadolinium-diethylenetriaminepentaacetic acid. All PET was acquired with a dedicated small-animal PET system. MRI was performed on a 1.5-T clinical tomograph with a dedicated small-animal electrocardiographic triggering device and a small surface coil. Results: In normal rats, 18F-FDG uptake was homogeneous throughout the left ventricle. The lowest mean uptake of the 18F-FDG was found in the apical regions (79{\%} ± 6.0{\%} of maximum) and the highest uptake was in the anterior wall (93{\%} ± 4.3 {\%} of maximum). Myocardial infarct size as determined by histology correlated well with defects of glucose metabolism obtained with 18F-FDG PET (r = 0.89) and also with delayed-enhancement MRI (r = 0.91). Left ventricular ejection fraction in normal rats measured by cine MRI was 57{\%} ± 5.4{\%} and decreased to 38{\%} ± 12.9{\%} (P <0.001) in the myocardial infarction model. Conclusion: Integrating information from small-animal PET and clinical MRI instrumentation allows for the quantitative assessment of cardiac function and infarct size in the rat model. The MRI measurements of scar can be complemented by metabolic imaging, addressing the extent and severity of ischemic injury and providing endpoints for therapeutic interventions.",
keywords = "MRI, Myocardial infarction, Rat, Small-animal PET",
author = "Takahiro Higuchi and Nekolla, {Stephan G.} and Antanas Jankaukas and Weber, {Axel W.} and Huisman, {Marc C.} and Sybille Reder and Ziegler, {Sibylle I.} and Markus Schwaiger and Bengel, {Frank M.}",
year = "2007",
month = "2",
day = "1",
language = "English (US)",
volume = "48",
pages = "288--294",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization of normal and infarcted rat myocardium using a combination of small-animal PET and clinical MRI

AU - Higuchi, Takahiro

AU - Nekolla, Stephan G.

AU - Jankaukas, Antanas

AU - Weber, Axel W.

AU - Huisman, Marc C.

AU - Reder, Sybille

AU - Ziegler, Sibylle I.

AU - Schwaiger, Markus

AU - Bengel, Frank M.

PY - 2007/2/1

Y1 - 2007/2/1

N2 - The combination of small-animal PET and MRI data provides quantitative in vivo insights into cardiac pathophysiology, integrating information on biology and morphology. We sought to determine the feasibility of PET and MRI for the quantification of ischemic injury in the rat model. Methods: Fourteen healthy male Wistar rats were studied with 18F-FDG PET and cine MRI. Myocardial viability was determined in a transmural myocardial infarction model in 12 additional rats, using 18F-FDG PET and delayed-enhancement MRI with gadolinium-diethylenetriaminepentaacetic acid. All PET was acquired with a dedicated small-animal PET system. MRI was performed on a 1.5-T clinical tomograph with a dedicated small-animal electrocardiographic triggering device and a small surface coil. Results: In normal rats, 18F-FDG uptake was homogeneous throughout the left ventricle. The lowest mean uptake of the 18F-FDG was found in the apical regions (79% ± 6.0% of maximum) and the highest uptake was in the anterior wall (93% ± 4.3 % of maximum). Myocardial infarct size as determined by histology correlated well with defects of glucose metabolism obtained with 18F-FDG PET (r = 0.89) and also with delayed-enhancement MRI (r = 0.91). Left ventricular ejection fraction in normal rats measured by cine MRI was 57% ± 5.4% and decreased to 38% ± 12.9% (P <0.001) in the myocardial infarction model. Conclusion: Integrating information from small-animal PET and clinical MRI instrumentation allows for the quantitative assessment of cardiac function and infarct size in the rat model. The MRI measurements of scar can be complemented by metabolic imaging, addressing the extent and severity of ischemic injury and providing endpoints for therapeutic interventions.

AB - The combination of small-animal PET and MRI data provides quantitative in vivo insights into cardiac pathophysiology, integrating information on biology and morphology. We sought to determine the feasibility of PET and MRI for the quantification of ischemic injury in the rat model. Methods: Fourteen healthy male Wistar rats were studied with 18F-FDG PET and cine MRI. Myocardial viability was determined in a transmural myocardial infarction model in 12 additional rats, using 18F-FDG PET and delayed-enhancement MRI with gadolinium-diethylenetriaminepentaacetic acid. All PET was acquired with a dedicated small-animal PET system. MRI was performed on a 1.5-T clinical tomograph with a dedicated small-animal electrocardiographic triggering device and a small surface coil. Results: In normal rats, 18F-FDG uptake was homogeneous throughout the left ventricle. The lowest mean uptake of the 18F-FDG was found in the apical regions (79% ± 6.0% of maximum) and the highest uptake was in the anterior wall (93% ± 4.3 % of maximum). Myocardial infarct size as determined by histology correlated well with defects of glucose metabolism obtained with 18F-FDG PET (r = 0.89) and also with delayed-enhancement MRI (r = 0.91). Left ventricular ejection fraction in normal rats measured by cine MRI was 57% ± 5.4% and decreased to 38% ± 12.9% (P <0.001) in the myocardial infarction model. Conclusion: Integrating information from small-animal PET and clinical MRI instrumentation allows for the quantitative assessment of cardiac function and infarct size in the rat model. The MRI measurements of scar can be complemented by metabolic imaging, addressing the extent and severity of ischemic injury and providing endpoints for therapeutic interventions.

KW - MRI

KW - Myocardial infarction

KW - Rat

KW - Small-animal PET

UR - http://www.scopus.com/inward/record.url?scp=34047238231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34047238231&partnerID=8YFLogxK

M3 - Article

VL - 48

SP - 288

EP - 294

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 2

ER -