Characterization of neuropathology in the HIV-1 transgenic rat at different ages

William C. Reid; Wael G. Ibrahim; Saejeong J. Kim; Frank Denaro; Rafael Casas; Dianne E. Lee; Dragan Maric; Dima A. Hammoud

doi:10.1016/j.jneuroim.2016.01.022

Characterization of neuropathology in the HIV-1 transgenic rat at different ages

William C. Reid, Wael G. Ibrahim, Saejeong J. Kim, Frank Denaro, Rafael Casas, Dianne E. Lee, Dragan Maric, Dima A. Hammoud

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The transgenic HIV-1 rat (Tg) is a commonly used neuroHIV model with documented neurologic/behavioral deficits. Using immunofluorescent staining of the Tg brain, we found astrocytic dysfunction/damage, as well as dopaminergic neuronal loss/dysfunction, both of which worsening significantly in the striatum with age. We saw mild microglial activation in young Tg brains, but this decreased with age. There were no differences in neurogenesis potential suggesting a neurodegenerative rather than a neurodevelopmental process. Gp120 CSF levels exceeded serum gp120 levels in some animals, suggesting local viral protein production in the brain. Further probing of the pathophysiology underlying astrocytic injury in this model is warranted.

Original language	English (US)
Pages (from-to)	116-125
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	292
DOIs	https://doi.org/10.1016/j.jneuroim.2016.01.022
State	Published - Mar 15 2016

Keywords

Astrocytic loss
HIV-1 transgenic rat
Neuro-HIV
Neuropathology
Neurotoxicity
Viral proteins

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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title = "Characterization of neuropathology in the HIV-1 transgenic rat at different ages",

abstract = "The transgenic HIV-1 rat (Tg) is a commonly used neuroHIV model with documented neurologic/behavioral deficits. Using immunofluorescent staining of the Tg brain, we found astrocytic dysfunction/damage, as well as dopaminergic neuronal loss/dysfunction, both of which worsening significantly in the striatum with age. We saw mild microglial activation in young Tg brains, but this decreased with age. There were no differences in neurogenesis potential suggesting a neurodegenerative rather than a neurodevelopmental process. Gp120 CSF levels exceeded serum gp120 levels in some animals, suggesting local viral protein production in the brain. Further probing of the pathophysiology underlying astrocytic injury in this model is warranted.",

keywords = "Astrocytic loss, HIV-1 transgenic rat, Neuro-HIV, Neuropathology, Neurotoxicity, Viral proteins",

author = "Reid, {William C.} and Ibrahim, {Wael G.} and Kim, {Saejeong J.} and Frank Denaro and Rafael Casas and Lee, {Dianne E.} and Dragan Maric and Hammoud, {Dima A.}",

note = "Funding Information: Sources of funding: This work was supported by the Center for Infectious Disease Imaging (CIDI), National Institutes of Health (Intramural Program) . Publisher Copyright: {\textcopyright} 2015 Published by Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2016",

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doi = "10.1016/j.jneuroim.2016.01.022",

language = "English (US)",

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AU - Ibrahim, Wael G.

AU - Kim, Saejeong J.

AU - Denaro, Frank

AU - Casas, Rafael

AU - Lee, Dianne E.

AU - Maric, Dragan

AU - Hammoud, Dima A.

N1 - Funding Information: Sources of funding: This work was supported by the Center for Infectious Disease Imaging (CIDI), National Institutes of Health (Intramural Program) . Publisher Copyright: © 2015 Published by Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - The transgenic HIV-1 rat (Tg) is a commonly used neuroHIV model with documented neurologic/behavioral deficits. Using immunofluorescent staining of the Tg brain, we found astrocytic dysfunction/damage, as well as dopaminergic neuronal loss/dysfunction, both of which worsening significantly in the striatum with age. We saw mild microglial activation in young Tg brains, but this decreased with age. There were no differences in neurogenesis potential suggesting a neurodegenerative rather than a neurodevelopmental process. Gp120 CSF levels exceeded serum gp120 levels in some animals, suggesting local viral protein production in the brain. Further probing of the pathophysiology underlying astrocytic injury in this model is warranted.

AB - The transgenic HIV-1 rat (Tg) is a commonly used neuroHIV model with documented neurologic/behavioral deficits. Using immunofluorescent staining of the Tg brain, we found astrocytic dysfunction/damage, as well as dopaminergic neuronal loss/dysfunction, both of which worsening significantly in the striatum with age. We saw mild microglial activation in young Tg brains, but this decreased with age. There were no differences in neurogenesis potential suggesting a neurodegenerative rather than a neurodevelopmental process. Gp120 CSF levels exceeded serum gp120 levels in some animals, suggesting local viral protein production in the brain. Further probing of the pathophysiology underlying astrocytic injury in this model is warranted.

KW - Astrocytic loss

KW - HIV-1 transgenic rat

KW - Neuro-HIV

KW - Neuropathology

KW - Neurotoxicity

KW - Viral proteins

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Characterization of neuropathology in the HIV-1 transgenic rat at different ages

Abstract

Keywords

ASJC Scopus subject areas

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