Characterization of muscarinic receptors in canine bronchial smooth muscle

Stephen T. O'Rourke, Nicholas A. Flavahan, Paul M. Vanhoutte

Research output: Contribution to journalArticle

Abstract

Rings of canine bronchi were suspended for isometric tension recording. Contractions produced by exogenously added acetylcholine were inhibited by pirenzepine and pancuronium. The pKB values were 6.76 for pirenzepine (calculated at 10-6 M) and 5.30 and 5.13 for pancuronium (calculated at 10-5 and 3 × 10-5 M, respectively). Contractile responses evoked by cholinergic nerve stimulation (0.2-16 Hz, 9 V) were depressed by pancuronium in a concentration-dependent manner, while concentrations of pirenzepine selective for M1-muscarinic receptors were without effect. The results indicate that exogenous and nerve-released acetylcholine activate a homogenous population of M2-muscarinic receptors in isolated preparations of canine bronchial smooth muscle.

Original languageEnglish (US)
Pages (from-to)117-120
Number of pages4
JournalEuropean Journal of Pharmacology
Volume140
Issue number1
DOIs
StatePublished - Aug 4 1987
Externally publishedYes

Keywords

  • (Canine)
  • Acetylcholine
  • Bronchi
  • Electrical stimulation
  • Muscarinic receptors
  • Pancuronium
  • Pirenzepine

ASJC Scopus subject areas

  • Pharmacology

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