Characterization of multiple myeloma vesicles by label-free relative quantitation

Sean W. Harshman, Alessandro Canella, Paul D. Ciarlariello, Alberto Rocci, Kitty Agarwal, Emily M. Smith, Tiffany Talabere, Yvonne A. Efebera, Craig C. Hofmeister, Don M. Benson, Michael E. Paulaitis, Michael A. Freitas, Flavia Pichiorri

Research output: Contribution to journalArticlepeer-review

Abstract

Multiple myeloma (MM) is a hematological malignancy caused by a microenviromentally aided persistence of plasma cells in the bone marrow. The role that extracellular vesicles (EVs), microvesicles and exosomes, released by MM cells have in cell-to-cell communication and signaling in the bone marrow is currently unknown. This paper describes the proteomic content of EVs derived from MM.1S and U266 MM cell lines. First, we compared the protein identifications between the vesicles and cellular lysates of each cell line finding a large overlap in protein identifications. Next, we applied label-free spectral count quantitation to determine proteins with differential abundance between the groups. Finally, we used bioinformatics to categorize proteins with significantly different abundances into functional groups. The results illustrate the first use of label-free spectral counting applied to determine relative protein abundances in EVs.

Original languageEnglish (US)
Pages (from-to)3013-3029
Number of pages17
JournalProteomics
Volume13
Issue number20
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

Keywords

  • Cell biology
  • Exosomes
  • LC-MS/MS
  • Label free
  • Microvesicles

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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