Characterization of multiple myeloma vesicles by label-free relative quantitation

Sean W. Harshman, Alessandro Canella, Paul D. Ciarlariello, Alberto Rocci, Kitty Agarwal, Emily M. Smith, Tiffany Talabere, Yvonne A. Efebera, Craig C. Hofmeister, Don M. Benson, Michael Paulaitis, Michael A. Freitas, Flavia Pichiorri

Research output: Contribution to journalArticle

Abstract

Multiple myeloma (MM) is a hematological malignancy caused by a microenviromentally aided persistence of plasma cells in the bone marrow. The role that extracellular vesicles (EVs), microvesicles and exosomes, released by MM cells have in cell-to-cell communication and signaling in the bone marrow is currently unknown. This paper describes the proteomic content of EVs derived from MM.1S and U266 MM cell lines. First, we compared the protein identifications between the vesicles and cellular lysates of each cell line finding a large overlap in protein identifications. Next, we applied label-free spectral count quantitation to determine proteins with differential abundance between the groups. Finally, we used bioinformatics to categorize proteins with significantly different abundances into functional groups. The results illustrate the first use of label-free spectral counting applied to determine relative protein abundances in EVs.

Original languageEnglish (US)
Pages (from-to)3013-3029
Number of pages17
JournalProteomics
Volume13
Issue number20
DOIs
StatePublished - Oct 2013
Externally publishedYes

Fingerprint

Multiple Myeloma
Labels
Proteins
Bone
Bone Marrow
Cells
Exosomes
Cell Line
Hematologic Neoplasms
Bioinformatics
Plasma Cells
Computational Biology
Cell Communication
Proteomics
Functional groups
Plasmas
Communication
Extracellular Vesicles

Keywords

  • Cell biology
  • Exosomes
  • Label free
  • LC-MS/MS
  • Microvesicles

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Harshman, S. W., Canella, A., Ciarlariello, P. D., Rocci, A., Agarwal, K., Smith, E. M., ... Pichiorri, F. (2013). Characterization of multiple myeloma vesicles by label-free relative quantitation. Proteomics, 13(20), 3013-3029. https://doi.org/10.1002/pmic.201300142

Characterization of multiple myeloma vesicles by label-free relative quantitation. / Harshman, Sean W.; Canella, Alessandro; Ciarlariello, Paul D.; Rocci, Alberto; Agarwal, Kitty; Smith, Emily M.; Talabere, Tiffany; Efebera, Yvonne A.; Hofmeister, Craig C.; Benson, Don M.; Paulaitis, Michael; Freitas, Michael A.; Pichiorri, Flavia.

In: Proteomics, Vol. 13, No. 20, 10.2013, p. 3013-3029.

Research output: Contribution to journalArticle

Harshman, SW, Canella, A, Ciarlariello, PD, Rocci, A, Agarwal, K, Smith, EM, Talabere, T, Efebera, YA, Hofmeister, CC, Benson, DM, Paulaitis, M, Freitas, MA & Pichiorri, F 2013, 'Characterization of multiple myeloma vesicles by label-free relative quantitation', Proteomics, vol. 13, no. 20, pp. 3013-3029. https://doi.org/10.1002/pmic.201300142
Harshman SW, Canella A, Ciarlariello PD, Rocci A, Agarwal K, Smith EM et al. Characterization of multiple myeloma vesicles by label-free relative quantitation. Proteomics. 2013 Oct;13(20):3013-3029. https://doi.org/10.1002/pmic.201300142
Harshman, Sean W. ; Canella, Alessandro ; Ciarlariello, Paul D. ; Rocci, Alberto ; Agarwal, Kitty ; Smith, Emily M. ; Talabere, Tiffany ; Efebera, Yvonne A. ; Hofmeister, Craig C. ; Benson, Don M. ; Paulaitis, Michael ; Freitas, Michael A. ; Pichiorri, Flavia. / Characterization of multiple myeloma vesicles by label-free relative quantitation. In: Proteomics. 2013 ; Vol. 13, No. 20. pp. 3013-3029.
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