TY - JOUR
T1 - Characterization of lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and yeast proteome microarrays
AU - Lu, Kuan Yi
AU - Chen, Chien Sheng
AU - Neiswinger, Johnathan
AU - Zhu, Heng
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Studying lipid-protein interactions is central to understanding lipid signaling, a key regulatory system in cells. To better identify lipid-binding proteins, we developed a nonquenched fluorescent (NQF) liposome that is able to carry both fluorescent molecules and a lipid of interest. By combining the strength of NQF liposomes with protein microarray technology, the method presented here facilitates high-throughput screening of lipid-protein interactions. This protocol describes how to prepare NQF liposomes and apply the fabricated liposomes to yeast proteome microarrays.
AB - Studying lipid-protein interactions is central to understanding lipid signaling, a key regulatory system in cells. To better identify lipid-binding proteins, we developed a nonquenched fluorescent (NQF) liposome that is able to carry both fluorescent molecules and a lipid of interest. By combining the strength of NQF liposomes with protein microarray technology, the method presented here facilitates high-throughput screening of lipid-protein interactions. This protocol describes how to prepare NQF liposomes and apply the fabricated liposomes to yeast proteome microarrays.
UR - http://www.scopus.com/inward/record.url?scp=84990038457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990038457&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot087981
DO - 10.1101/pdb.prot087981
M3 - Article
C2 - 27698237
AN - SCOPUS:84990038457
SN - 1940-3402
VL - 2016
SP - 887
EP - 891
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -