Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton

Tara L. Lauriat, Stella Dracheva, Joachim Kremerskothen, Kerstin Duning, Vahram Haroutunian, Joseph D. Buxbaum, Thomas Hyde, Joel Kleinman, L. Alison McInnes

Research output: Contribution to journalArticle

Abstract

KIAA0513 was previously identified as upregulated in the dorsolateral prefrontal cortex of subjects with schizophrenia by microarray analysis. In the present study, the differential expression in the schizophrenic subjects was confirmed by quantitative RT-PCR. The limited homology to proteins of known function and lack of functional domains in the encoded protein have made it difficult to predict a function for KIAA0513. We used in situ hybridization, RNA blots, western blots, and immunocytochemistry to examine KIAA0513 expression in normal brain and peripheral tissues. The gene is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. Finally, interacting proteins were identified using a yeast two-hybrid screen to functionally characterize the protein. KIAA0513 interacts with KIBRA, HAX-1, and INTS4, which also interact with proteins involved in neuroplasticity, apoptosis, and cytoskeletal regulation. Therefore, KIAA0513 is likely to be involved in signaling pathways related to these processes.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalBrain Research
Volume1121
Issue number1
DOIs
StatePublished - Nov 22 2006
Externally publishedYes

Fingerprint

Neuronal Plasticity
Cytoskeleton
Apoptosis
Proteins
Brain
Microarray Analysis
Prefrontal Cortex
Cerebellum
In Situ Hybridization
Schizophrenia
Yeasts
Western Blotting
Immunohistochemistry
RNA
Polymerase Chain Reaction
Genes

Keywords

  • Cerebellum
  • Gene expression
  • KIBRA
  • RT-PCR
  • Schizophrenia
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Lauriat, T. L., Dracheva, S., Kremerskothen, J., Duning, K., Haroutunian, V., Buxbaum, J. D., ... Alison McInnes, L. (2006). Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton. Brain Research, 1121(1), 1-11. https://doi.org/10.1016/j.brainres.2006.08.099

Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton. / Lauriat, Tara L.; Dracheva, Stella; Kremerskothen, Joachim; Duning, Kerstin; Haroutunian, Vahram; Buxbaum, Joseph D.; Hyde, Thomas; Kleinman, Joel; Alison McInnes, L.

In: Brain Research, Vol. 1121, No. 1, 22.11.2006, p. 1-11.

Research output: Contribution to journalArticle

Lauriat, Tara L. ; Dracheva, Stella ; Kremerskothen, Joachim ; Duning, Kerstin ; Haroutunian, Vahram ; Buxbaum, Joseph D. ; Hyde, Thomas ; Kleinman, Joel ; Alison McInnes, L. / Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton. In: Brain Research. 2006 ; Vol. 1121, No. 1. pp. 1-11.
@article{b5ab884c2f2f454bb08a8fa6de45e547,
title = "Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton",
abstract = "KIAA0513 was previously identified as upregulated in the dorsolateral prefrontal cortex of subjects with schizophrenia by microarray analysis. In the present study, the differential expression in the schizophrenic subjects was confirmed by quantitative RT-PCR. The limited homology to proteins of known function and lack of functional domains in the encoded protein have made it difficult to predict a function for KIAA0513. We used in situ hybridization, RNA blots, western blots, and immunocytochemistry to examine KIAA0513 expression in normal brain and peripheral tissues. The gene is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. Finally, interacting proteins were identified using a yeast two-hybrid screen to functionally characterize the protein. KIAA0513 interacts with KIBRA, HAX-1, and INTS4, which also interact with proteins involved in neuroplasticity, apoptosis, and cytoskeletal regulation. Therefore, KIAA0513 is likely to be involved in signaling pathways related to these processes.",
keywords = "Cerebellum, Gene expression, KIBRA, RT-PCR, Schizophrenia, Yeast two-hybrid",
author = "Lauriat, {Tara L.} and Stella Dracheva and Joachim Kremerskothen and Kerstin Duning and Vahram Haroutunian and Buxbaum, {Joseph D.} and Thomas Hyde and Joel Kleinman and {Alison McInnes}, L.",
year = "2006",
month = "11",
day = "22",
doi = "10.1016/j.brainres.2006.08.099",
language = "English (US)",
volume = "1121",
pages = "1--11",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Characterization of KIAA0513, a novel signaling molecule that interacts with modulators of neuroplasticity, apoptosis, and the cytoskeleton

AU - Lauriat, Tara L.

AU - Dracheva, Stella

AU - Kremerskothen, Joachim

AU - Duning, Kerstin

AU - Haroutunian, Vahram

AU - Buxbaum, Joseph D.

AU - Hyde, Thomas

AU - Kleinman, Joel

AU - Alison McInnes, L.

PY - 2006/11/22

Y1 - 2006/11/22

N2 - KIAA0513 was previously identified as upregulated in the dorsolateral prefrontal cortex of subjects with schizophrenia by microarray analysis. In the present study, the differential expression in the schizophrenic subjects was confirmed by quantitative RT-PCR. The limited homology to proteins of known function and lack of functional domains in the encoded protein have made it difficult to predict a function for KIAA0513. We used in situ hybridization, RNA blots, western blots, and immunocytochemistry to examine KIAA0513 expression in normal brain and peripheral tissues. The gene is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. Finally, interacting proteins were identified using a yeast two-hybrid screen to functionally characterize the protein. KIAA0513 interacts with KIBRA, HAX-1, and INTS4, which also interact with proteins involved in neuroplasticity, apoptosis, and cytoskeletal regulation. Therefore, KIAA0513 is likely to be involved in signaling pathways related to these processes.

AB - KIAA0513 was previously identified as upregulated in the dorsolateral prefrontal cortex of subjects with schizophrenia by microarray analysis. In the present study, the differential expression in the schizophrenic subjects was confirmed by quantitative RT-PCR. The limited homology to proteins of known function and lack of functional domains in the encoded protein have made it difficult to predict a function for KIAA0513. We used in situ hybridization, RNA blots, western blots, and immunocytochemistry to examine KIAA0513 expression in normal brain and peripheral tissues. The gene is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. Finally, interacting proteins were identified using a yeast two-hybrid screen to functionally characterize the protein. KIAA0513 interacts with KIBRA, HAX-1, and INTS4, which also interact with proteins involved in neuroplasticity, apoptosis, and cytoskeletal regulation. Therefore, KIAA0513 is likely to be involved in signaling pathways related to these processes.

KW - Cerebellum

KW - Gene expression

KW - KIBRA

KW - RT-PCR

KW - Schizophrenia

KW - Yeast two-hybrid

UR - http://www.scopus.com/inward/record.url?scp=33750724136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750724136&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.08.099

DO - 10.1016/j.brainres.2006.08.099

M3 - Article

C2 - 17010949

AN - SCOPUS:33750724136

VL - 1121

SP - 1

EP - 11

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -