TY - JOUR
T1 - Characterization of HIV type 1 subtype C protease gene
T2 - Selection of L63P mutation in protease inhibitor-naive Indian patients
AU - Neogi, Ujjwal
AU - Sahoo, Pravat Nalini
AU - Kumar, Ravi
AU - De Costa, Ayesha
AU - Shet, Anita
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Significant subtype-specific differences were observed in the protease (PR) region of the HIV-1 pol gene. Most of the previous studies were restricted to subtype B, although subtype C accounts for more than 50% of HIV infections worldwide. In this study we characterized PR sequences from primary clinical isolates from protease inhibitor (PI)-naive patients in South India (n=39) as well as database-derived HIV-1 subtype C sequences from India (n=542) and globally (n=2970). All the study sequences were identified as subtype C, which is predominant in India. Drug resistance genotyping analysis identified 2.6% (1/39) prevalence of major PI resistance (I54T) and 7.7% (3/39) of minor PI resistance (L10I, T74S, and A71T). Selection of T12S, I15V, L19I, M36I, R41K, H69K, L89M, and I93L was observed both in global and Indian subtype C while the L63P mutation was selected in Indian PR sequences. Three different codon-based maximum likelihood methods agreed on four sites (12, 19, 36, and 82) under positive selection in Indian sequences.
AB - Significant subtype-specific differences were observed in the protease (PR) region of the HIV-1 pol gene. Most of the previous studies were restricted to subtype B, although subtype C accounts for more than 50% of HIV infections worldwide. In this study we characterized PR sequences from primary clinical isolates from protease inhibitor (PI)-naive patients in South India (n=39) as well as database-derived HIV-1 subtype C sequences from India (n=542) and globally (n=2970). All the study sequences were identified as subtype C, which is predominant in India. Drug resistance genotyping analysis identified 2.6% (1/39) prevalence of major PI resistance (I54T) and 7.7% (3/39) of minor PI resistance (L10I, T74S, and A71T). Selection of T12S, I15V, L19I, M36I, R41K, H69K, L89M, and I93L was observed both in global and Indian subtype C while the L63P mutation was selected in Indian PR sequences. Three different codon-based maximum likelihood methods agreed on four sites (12, 19, 36, and 82) under positive selection in Indian sequences.
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U2 - 10.1089/aid.2011.0078
DO - 10.1089/aid.2011.0078
M3 - Article
C2 - 21453185
AN - SCOPUS:80655128320
SN - 0889-2229
VL - 27
SP - 1249
EP - 1253
JO - AIDS research and human retroviruses
JF - AIDS research and human retroviruses
IS - 11
ER -