Characterization of early neovascular response to acute lung ischemia using simultaneous 19F/1H MR molecular imaging

Anne H. Schmieder, Kezheng Wang, Huiying Zhang, Angana Senpan, Dipanjan Pan, Jochen Keupp, Shelton D. Caruthers, Samuel A. Wickline, Baozhong Shen, Elizabeth Marie Wagner, Gregory M. Lanza

Research output: Contribution to journalArticle

Abstract

Angiogenesis is an important constituent of many inflammatory pulmonary diseases, which has been unappreciated until recently. Early neovascular expansion in the lungs in preclinical models and patients is very difficult to assess noninvasively, particularly quantitatively. The present study demonstrated that 19F/1H MR molecular imaging with αvβ3-targeted perfluorocarbon nanoparticles can be used to directly measure neovascularity in a rat left pulmonary artery ligation (LPAL) model, which was employed to create pulmonary ischemia and induce angiogenesis. In rats 3 days after LPAL, simultaneous 19F/1H MR imaging at 3T revealed a marked 19F signal in animals 2 h following αvβ3-targeted perfluorocarbon nanoparticles [19F signal (normalized to background) = 0.80 ± 0.2] that was greater (p = 0.007) than the non-targeted (0.30 ± 0.04) and the sham-operated (0.07 ± 0.09) control groups. Almost no 19F signal was found in control right lung with any treatment. Competitive blockade of the integrin-targeted particles greatly decreased the 19F signal (p = 0.002) and was equivalent to the non-targeted control group. Fluorescent and light microscopy illustrated heavy decorating of vessel walls in and around large bronchi and large pulmonary vessels. Focal segmental regions of neovessel expansion were also noted in the lung periphery. Our results demonstrate that 19F/1H MR molecular imaging with αvβ3-targeted perfluorocarbon nanoparticles provides a means to assess the extent of systemic neovascularization in the lung.

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalAngiogenesis
Volume17
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Molecular imaging
Fluorocarbons
Molecular Imaging
Ischemia
Nanoparticles
Lung
Rats
Pulmonary diseases
Integrins
Pulmonary Artery
Optical microscopy
Ligation
Animals
Imaging techniques
Control Groups
Bronchi
Lung Diseases
Microscopy
Light

Keywords

  • Angiogenesis
  • Fluorine
  • Lung
  • Molecular imaging
  • MRI
  • Nanotechnology

ASJC Scopus subject areas

  • Cancer Research
  • Physiology
  • Clinical Biochemistry

Cite this

Schmieder, A. H., Wang, K., Zhang, H., Senpan, A., Pan, D., Keupp, J., ... Lanza, G. M. (2014). Characterization of early neovascular response to acute lung ischemia using simultaneous 19F/1H MR molecular imaging. Angiogenesis, 17(1), 51-60. https://doi.org/10.1007/s10456-013-9377-2

Characterization of early neovascular response to acute lung ischemia using simultaneous 19F/1H MR molecular imaging. / Schmieder, Anne H.; Wang, Kezheng; Zhang, Huiying; Senpan, Angana; Pan, Dipanjan; Keupp, Jochen; Caruthers, Shelton D.; Wickline, Samuel A.; Shen, Baozhong; Wagner, Elizabeth Marie; Lanza, Gregory M.

In: Angiogenesis, Vol. 17, No. 1, 01.2014, p. 51-60.

Research output: Contribution to journalArticle

Schmieder, AH, Wang, K, Zhang, H, Senpan, A, Pan, D, Keupp, J, Caruthers, SD, Wickline, SA, Shen, B, Wagner, EM & Lanza, GM 2014, 'Characterization of early neovascular response to acute lung ischemia using simultaneous 19F/1H MR molecular imaging', Angiogenesis, vol. 17, no. 1, pp. 51-60. https://doi.org/10.1007/s10456-013-9377-2
Schmieder, Anne H. ; Wang, Kezheng ; Zhang, Huiying ; Senpan, Angana ; Pan, Dipanjan ; Keupp, Jochen ; Caruthers, Shelton D. ; Wickline, Samuel A. ; Shen, Baozhong ; Wagner, Elizabeth Marie ; Lanza, Gregory M. / Characterization of early neovascular response to acute lung ischemia using simultaneous 19F/1H MR molecular imaging. In: Angiogenesis. 2014 ; Vol. 17, No. 1. pp. 51-60.
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