Characterization of complementary determinant region 3δ in human MutS homologue 2-specific γδ T Cells

H. Chen, X. Ji, L. Cui, J. Zhang, W. He

Research output: Contribution to journalArticle

Abstract

γδT cells function as sentinels in early host responses to infections and malignancies. Previously, we found ectopically expressed human MutS homologue 2 (hMSH2), recognized by γδT cells, triggered a γδT cell-mediated cytolysis to tumor cells. However, the characteristics of hMSH2-specific γδ Τ cells are not fully understood. In this study, we investigated the complementary determinant region (CDR) 3δ diversity of hMSH2-specific γδ T cells. We found that the CDR3δ sequences of hMSH2-specific γδ T cells displayed limited diversity, while the length and germline gene usage showed no differences compared with whole CDR3δ immune repertoire. There are more hydrophilic amino acids in P/N insert of hMSH2-specific γδ T cells including the more conserved amino acid at the position 97. Our results offer clues to understanding antigen recognition pattern of γδ T cells to stress-induced hMSH2 of tumor cells and also the mechanism of γδT cell-mediated tumor immune surveillance.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalScandinavian Journal of Immunology
Volume81
Issue number2
DOIs
StatePublished - Feb 1 2015

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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