Characterization of canine adipose tissue-derived mesenchymal stem cells immortalized by SV40-T retrovirus for therapeutic use

Ana Patricia Ayala-Cuellar, Cho Won Kim, Kyung A. Hwang, Ji Houn Kang, Gabsang Lee, Jaejin Cho, Kyung Chul Choi

Research output: Contribution to journalArticlepeer-review

Abstract

Canine mesenchymal stem cells (cMSCs) are gaining popularity in the veterinary field as a regenerative therapy. But, their limited culture lifespan makes it an obstacle for preclinical investigation and therapeutic use. In this study, primary canine adipose tissue-derived MSCs (PCAT-MSCs) were isolated from adipose tissue and were transfected with the SV40-T retrovirus resulting in a life-extended immortalized canine adipose tissue-derived MSCs (ICAT-MSCs). A comparison was made through the characterization of both PCAT-MSCs and ICAT-MSCs. Both showed a fibroblastic morphology; ICAT-MSCs showed a higher potential of colony formation compared with PCAT-MSCs and a reduced population doubling time; stem cell markers SOX2 and NANOG were expressed in both cell lines; karyotyping analysis showed no abnormalities in both PCAT-MSCs and ICAT-MSCs; both cell lines were CD90+, CD44 +, and CD45 ; both generated chondrogenic pellet; in osteogenic differentiation both showed upregulation of Osterix, a master transcriptome of osteogenesis, but in PCAT-MSCs, an upregulation of SOX2 was also observed. In conclusion, ICAT-MSCs showed similar characteristics with PCAT-MSCs, thus established as an easy to access platform for studies on better understanding about cMSCs nature.

Original languageEnglish (US)
Pages (from-to)16630-16642
Number of pages13
JournalJournal of Cellular Physiology
Volume234
Issue number9
DOIs
StatePublished - Sep 2019

Keywords

  • SV40-T
  • canine stem cells
  • differentiation
  • mesenchymal stem cells
  • osteogenesis

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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