TY - JOUR
T1 - Characterization of Campylobacter-like organisms isolated from homosexual men
AU - Fennell, C. L.
AU - Totten, P. A.
AU - Quinn, T. C.
AU - Patton, D. L.
AU - Holmes, K. K.
AU - Stamm, W. E.
N1 - Funding Information:
Received for publication May 25, 1983, and in revised form August 12, 1983. This study was supported by grants AI-17805 and AI-12192 from the National Institutes of Health. We thank Sydney Harvey (Irvine Diagnostics, Irvine, California) for providing strains of Campylobacter species and JoAnn Gates (Veterans Administration Hospital, Seattle, Washington) for performing the transmission electron microscopy. Please address requests for reprints to Dr Walter E. Stamm, Department of Medicine, Harborview Medical Center, 325 9th Avenue, Seattle, Washington 98104. *Present address: Department of Medicine, The Johns Hopkins Medical School, Baltimore, Maryland.
PY - 1984
Y1 - 1984
N2 - Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-μg disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.
AB - Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-μg disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.
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U2 - 10.1093/infdis/149.1.58
DO - 10.1093/infdis/149.1.58
M3 - Article
C2 - 6693790
AN - SCOPUS:0021336676
SN - 0022-1899
VL - 149
SP - 58
EP - 66
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -