Characterization of AziR, a resistance protein of the DNA cross-linking agent azinomycin B

Jennifer Foulke-Abel, Gilbert T. Kelly, Huitu Zhang, Coran M.H. Watanabe

Research output: Contribution to journalArticlepeer-review

Abstract

A protein identified from the Streptomyces sahachiroi genome exhibits a protective effect against the DNA alkylator azinomycin B when heterologously expressed in S. lividans and E. coli. The protein, dubbed AziR for azinomycin resistance, is homologous to aminoglycoside phosphotransferases but behaves as an azinomycin binding protein and fails to chemically modify azinomycin. While AziR confers resistance to azinomycin B, it is inactive against aminoglycoside antibiotics and other DNA alkylators. A nucleic acid staining assay indicates that the protein enhances cell survival, and also prevents DNA damage effects normally observed following azinomycin treatment. Knowledge of an azinomycin resistance mechanism aids in setting the stage for future engineered biosynthesis of functionally useful azinomycin analogues.

Original languageEnglish (US)
Pages (from-to)2563-2570
Number of pages8
JournalMolecular BioSystems
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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